Liu Longwei, Wan Peng, Li Yang, Duan Zhiwen, Peng Cheng, Yuan Shuzhong, Deng Wei
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225109, China.
Plants (Basel). 2022 Jul 25;11(15):1926. doi: 10.3390/plants11151926.
is a troublesome broadleaf weed, widely distributed in the paddy fields of southern China. In this study, 10 biotypes of were sampled from Yangzhou City, China, where the paddy fields were seriously infested with , and their resistance levels to acetolactate synthase (ALS) inhibitor bensulfuron-methyl were determined. The whole-plant response assays showed that nine biotypes were highly resistant (from 16.4- to 183.1-fold) to bensulfuron-methyl in comparison with a susceptible YZ-S biotype, and only one YZ-6 biotype was susceptible. gene sequencing revealed that three gene copies existed in , and four different amino acid substitutions (Pro197-Leu, -Ala, -Ser, and -His) at site 197 in the or genes were found in eight resistant biotypes. In addition, no amino acid mutations in three genes were found in the YZ-3 biotype. These results suggested that target-site mutations or non-target-site resistance mechanisms were involved in tested resistant biotypes. Finally, a cleaved amplified polymorphic sequence (CAPS) marker was identified to rapidly detect the Pro197 mutations in .
是一种 troublesome 阔叶杂草，广泛分布于中国南方的稻田。在本研究中，从中国扬州市采集了 10 种该杂草的生物型样本，当地稻田中该杂草严重泛滥，并测定了它们对乙酰乳酸合成酶（ALS）抑制剂苄嘧磺隆的抗性水平。整株反应试验表明，与敏感的 YZ - S 生物型相比，9 种该杂草生物型对苄嘧磺隆具有高抗性（抗性倍数为 16.4 至 183.1 倍），只有一种 YZ - 6 生物型敏感。基因测序显示该杂草存在三个该基因拷贝，并且在八个抗性生物型的该基因或该基因的第 197 位点发现了四个不同的氨基酸替换（Pro197 - Leu、 - Ala、 - Ser 和 - His）。此外，在 YZ - 3 生物型的三个该基因中未发现氨基酸突变。这些结果表明，目标位点突变或非目标位点抗性机制参与了测试的抗性该杂草生物型。最后，鉴定出一种酶切扩增多态性序列（CAPS）标记，用于快速检测该杂草中 Pro197 突变。
