Lan Yuning, Zhou Xinxin, Lin Shenyuan, Cao Yi, Wei Shouhui, Huang Hongjuan, Li Wenyu, Huang Zhaofeng
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Institute Control of Agrochemicals, Ministry of Agriculture and Rural Affairs, Beijing 100125, China.
Plants (Basel). 2022 Jun 21;11(13):1641. doi: 10.3390/plants11131641.
In crop fields, resistance to acetolactate synthase (ALS)-inhibiting herbicides found in many troublesome weed species, including Thunb, is a worldwide problem. In particular, the development of herbicide resistance in is a severe threat to wheat production in China. The purpose of this research was to investigate the physiological and molecular basis of resistance to flucarbazone-sodium. Dose-response analysis demonstrated that, compared with the susceptible (S) population, the resistant (R) population exhibited a 120-fold increase in flucarbazone-sodium resistance. Nucleotide sequence alignment of the ALS gene indicated that the Pro-197-Ser mutation in ALS was associated with resistance to flucarbazone-sodium in the R population. The results of a malathion pretreatment study showed that might also have remarkable cytochrome P450 monooxygenase (P450)-mediated metabolic resistance. This is the first report of a Pro-197-Ser mutation and P450-mediated metabolism conferring resistance to flucarbazone-sodium in .
在农田中，许多棘手杂草物种（包括[具体物种名称未给出]）对乙酰乳酸合成酶（ALS）抑制型除草剂产生抗性是一个全球性问题。特别是[具体物种名称未给出]中除草剂抗性的发展对中国的小麦生产构成严重威胁。本研究的目的是探究[具体物种名称未给出]对氟唑磺隆抗性的生理和分子基础。剂量反应分析表明，与敏感的[具体物种名称未给出]（S）种群相比，抗性（R）种群对氟唑磺隆的抗性增加了120倍。ALS基因的核苷酸序列比对表明，ALS中Pro-197-Ser突变与R种群对氟唑磺隆的抗性有关。马拉硫磷预处理研究结果表明，[具体物种名称未给出]可能还具有显著的细胞色素P450单加氧酶（P450）介导的代谢抗性。这是关于Pro-197-Ser突变和P450介导的代谢赋予[具体物种名称未给出]对氟唑磺隆抗性的首次报道。
