• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

来自……的铁调素同源物CreFH1的结构与功能表征

Structural and Functional Characterization of CreFH1, the Frataxin Homolog from .

作者信息

Terenzi Agustina, Pagani Maria A, Gomez-Casati Diego F, Busi Maria V

机构信息

Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Rosario 2000, Argentina.

出版信息

Plants (Basel). 2022 Jul 26;11(15):1931. doi: 10.3390/plants11151931.

DOI:10.3390/plants11151931
PMID:35893635
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9331050/
Abstract

Frataxin plays a key role in cellular iron homeostasis of different organisms. It has been implicated in iron storage, detoxification, delivery for Fe-S cluster assembly and heme biosynthesis. However, its specific role in iron metabolism remains unclear, especially in photosynthetic organisms. To gain insight into the role and properties of frataxin in algae, we identified the gene CreFH1, which codes for the frataxin homolog from . We performed the cloning, expression and biochemical characterization of CreFH1. This protein has a predicted mitochondrial transit peptide and a significant structural similarity to other members of the frataxin family. In addition, CreFH1 was able to form a dimer in vitro, and this effect was increased by the addition of Cu and also attenuated the Fenton reaction in the presence of a mixture of Fe and HO. Bacterial cells with overexpression of CreFH1 showed increased growth in the presence of different metals, such as Fe, Cu, Zn and Ni and HO. Thus, results indicated that CreFH1 is a functional protein that shows some distinctive features compared to its more well-known counterparts, and would play an important role in response to oxidative stress in

摘要

铁调素在不同生物体的细胞铁稳态中起着关键作用。它与铁储存、解毒、铁硫簇组装的铁传递以及血红素生物合成有关。然而，其在铁代谢中的具体作用仍不清楚，尤其是在光合生物中。为了深入了解铁调素在藻类中的作用和特性，我们鉴定了基因CreFH1，它编码来自……的铁调素同源物。我们对CreFH1进行了克隆、表达及生化特性分析。该蛋白具有预测的线粒体转运肽，与铁调素家族的其他成员有显著的结构相似性。此外，CreFH1能够在体外形成二聚体，添加铜可增强这种作用，并且在铁和过氧化氢混合物存在的情况下减弱芬顿反应。过表达CreFH1的细菌细胞在存在不同金属（如铁、铜、锌和镍）以及过氧化氢的情况下生长增加。因此，结果表明CreFH1是一种功能性蛋白，与其更为人熟知的同类蛋白相比具有一些独特特征，并且在应对……中的氧化应激时将发挥重要作用

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/675bf87831f6/plants-11-01931-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/51ada72de4ea/plants-11-01931-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/12f7a8f38404/plants-11-01931-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/38e6960cd0ad/plants-11-01931-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/e0345921219b/plants-11-01931-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/184518d362ec/plants-11-01931-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/a92c97aa89c4/plants-11-01931-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/8a6c5b1190fa/plants-11-01931-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/675bf87831f6/plants-11-01931-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/51ada72de4ea/plants-11-01931-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/12f7a8f38404/plants-11-01931-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/38e6960cd0ad/plants-11-01931-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/e0345921219b/plants-11-01931-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/184518d362ec/plants-11-01931-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/a92c97aa89c4/plants-11-01931-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/8a6c5b1190fa/plants-11-01931-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a39/9331050/675bf87831f6/plants-11-01931-g008.jpg

相似文献

1
Structural and Functional Characterization of CreFH1, the Frataxin Homolog from .来自……的铁调素同源物CreFH1的结构与功能表征
Plants (Basel). 2022 Jul 26;11(15):1931. doi: 10.3390/plants11151931.
2
Expression and one-step purification of recombinant Arabidopsis thaliana frataxin homolog (AtFH).重组拟南芥铁转运蛋白同源物（AtFH）的表达与一步纯化
Protein Expr Purif. 2007 Feb;51(2):157-61. doi: 10.1016/j.pep.2006.06.007. Epub 2006 Jun 22.
3
The role of frataxin in fission yeast iron metabolism: implications for Friedreich's ataxia.酵母铁蛋白在裂殖酵母铁代谢中的作用：对弗里德赖希共济失调的启示
Biochim Biophys Acta. 2014 Oct;1840(10):3022-33. doi: 10.1016/j.bbagen.2014.06.017. Epub 2014 Jul 3.
4
The Structure of the Complex between Yeast Frataxin and Ferrochelatase: CHARACTERIZATION AND PRE-STEADY STATE REACTION OF FERROUS IRON DELIVERY AND HEME SYNTHESIS.酵母铁调素与亚铁螯合酶复合物的结构：亚铁传递和血红素合成的表征及稳态前反应
J Biol Chem. 2016 May 27;291(22):11887-98. doi: 10.1074/jbc.M115.701128. Epub 2016 Mar 29.
5
The mitochondrial protein frataxin is essential for heme biosynthesis in plants.线粒体蛋白 frataxin 是植物血红素生物合成所必需的。
FEBS J. 2011 Feb;278(3):470-81. doi: 10.1111/j.1742-4658.2010.07968.x. Epub 2010 Dec 17.
6
Overexpression of the yeast frataxin homolog (Yfh1): contrasting effects on iron-sulfur cluster assembly, heme synthesis and resistance to oxidative stress.酵母铁转运蛋白同源物（Yfh1）的过表达：对铁硫簇组装、血红素合成及抗氧化应激能力的不同影响
Mitochondrion. 2009 Apr;9(2):130-8. doi: 10.1016/j.mito.2009.01.007. Epub 2009 Jan 22.
7
Erythroid differentiation and protoporphyrin IX down-regulate frataxin expression in Friend cells: characterization of frataxin expression compared to molecules involved in iron metabolism and hemoglobinization.红细胞分化和原卟啉IX下调弗拉塔xin在Friend细胞中的表达：与铁代谢和血红蛋白化相关分子相比弗拉塔xin表达的特征
Blood. 2002 May 15;99(10):3813-22. doi: 10.1182/blood.v99.10.3813.
8
The yeast frataxin homolog Yfh1p plays a specific role in the maturation of cellular Fe/S proteins.酵母中的铁调素同源物Yfh1p在细胞铁硫蛋白的成熟过程中发挥特定作用。
Hum Mol Genet. 2002 Aug 15;11(17):2025-36. doi: 10.1093/hmg/11.17.2025.
9
Mechanisms of iron and copper-frataxin interactions.铁和铜-铁叶啉相互作用的机制。
Metallomics. 2017 Aug 16;9(8):1073-1085. doi: 10.1039/c7mt00031f.
10
Functional studies of frataxin.弗里德赖希共济失调蛋白的功能研究
Acta Paediatr Suppl. 2004 May;93(445):68-71; discussion 72-3. doi: 10.1111/j.1651-2227.2004.tb03061.x.

引用本文的文献

1
Frataxin: from the sequence to the biological role.铁调素：从序列到生物学作用
Biophys Rev. 2025 Apr 3;17(2):449-465. doi: 10.1007/s12551-025-01311-z. eCollection 2025 Apr.

本文引用的文献

1
Fe-S Protein Synthesis in Green Algae Mitochondria.绿藻线粒体中Fe-S蛋白的合成
Plants (Basel). 2021 Jan 21;10(2):200. doi: 10.3390/plants10020200.
2
Iron-Sulfur Cluster Complex Assembly in the Mitochondria of .……线粒体中的铁硫簇复合物组装
Plants (Basel). 2020 Sep 9;9(9):1171. doi: 10.3390/plants9091171.
3
Identification of Frataxin as a regulator of ferroptosis.鉴定铁蛋白为铁死亡的调控因子。
Redox Biol. 2020 May;32:101483. doi: 10.1016/j.redox.2020.101483. Epub 2020 Mar 2.
4
Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism.人源 frataxin 结合的铁硫簇组装复合物的结构为其激活机制提供了线索。
Nat Commun. 2019 May 17;10(1):2210. doi: 10.1038/s41467-019-09989-y.
5
Altered levels of mitochondrial NFS1 affect cellular Fe and S contents in plants.线粒体 NFS1 水平的改变会影响植物细胞内的铁和硫含量。
Plant Cell Rep. 2019 Aug;38(8):981-990. doi: 10.1007/s00299-019-02419-9. Epub 2019 May 7.
6
Plant Frataxin in Metal Metabolism.植物中的铁调素与金属代谢
Front Plant Sci. 2018 Nov 21;9:1706. doi: 10.3389/fpls.2018.01706. eCollection 2018.
7
Ferrochelatase activity of plant frataxin.植物铁螯合酶的活性。
Biochimie. 2019 Jan;156:118-122. doi: 10.1016/j.biochi.2018.10.009. Epub 2018 Oct 18.
8
Interactions of iron-bound frataxin with ISCU and ferredoxin on the cysteine desulfurase complex leading to Fe-S cluster assembly.铁结合的 frataxin 与 ISCU 和铁氧还蛋白在半胱氨酸脱硫酶复合物上的相互作用导致 Fe-S 簇的组装。
J Inorg Biochem. 2018 Jun;183:107-116. doi: 10.1016/j.jinorgbio.2018.03.007. Epub 2018 Mar 15.
9
Copper redox chemistry of plant frataxins.植物 frataxins 的铜氧化还原化学。
J Inorg Biochem. 2018 Mar;180:135-140. doi: 10.1016/j.jinorgbio.2017.11.020. Epub 2017 Nov 27.
10
Iron-induced oligomerization of human FXN81-210 and bacterial CyaY frataxin and the effect of iron chelators.铁诱导的人 FXN81-210 和细菌 CyaY 铁蛋白的寡聚化及铁螯合剂的影响。
PLoS One. 2017 Dec 4;12(12):e0188937. doi: 10.1371/journal.pone.0188937. eCollection 2017.