Xin Aiyi, Jin Hui, Yang Xiaoyan, Guan Jinfeng, Hui Heping, Liu Haoyue, Cui Zengtuan, Dun Zhiheng, Qin Bo
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, China.
School of Chemistry and Chemical Engineering, Mianyang Normal University, Mianyang 621000, China.
Plants (Basel). 2022 Jul 26;11(15):1934. doi: 10.3390/plants11151934.
To reveal the allelopathic effects of potato, seven compounds were isolated from the rhizosphere soil: 7-methoxycoumarin (), palmitic acid (), caffeic acid (), chlorogenic acid (), quercetin dehydrate (), quercitrin (), and rutin (). Bioassays showed that compounds , , , and had inhibitory effects on the growth of and tissue culture seedlings of potato. The existence of the allelochemicals was confirmed by HPLC, and their contents were quantified with a total concentration of 9.02 μg/g in the rhizosphere soil of replanted potato. Approaches on the interactions of the allelochemicals and pathogens of potato including , , , , , and revealed that compound had inhibitory effects but compounds - promoted the colony growth of the pathogens. These findings demonstrated that the autotoxic allelopathy and enhancement of the pathogens caused by the accumulation of the allelochemicals in the continuously cropped soil should be one of the main reasons for the replant problems of potato.
为揭示马铃薯的化感作用，从根际土壤中分离出7种化合物：7-甲氧基香豆素、棕榈酸、咖啡酸、绿原酸、槲皮素脱水物、槲皮苷和芦丁。生物测定表明，化合物、、和对马铃薯的生长以及组培苗有抑制作用。通过高效液相色谱法（HPLC）证实了化感物质的存在，并对其含量进行了定量，连作马铃薯根际土壤中总浓度为9.02μg/g。对化感物质与马铃薯病原体（包括、、、、和）相互作用的研究表明，化合物有抑制作用，而化合物 - 促进了病原体的菌落生长。这些发现表明，化感物质在连作土壤中的积累导致的自毒化感作用和病原体的增强应是马铃薯连作问题的主要原因之一。
