Pham Lan Doan, Ba Nguyen Van, Nam Le Quang, Tuan Phong Vuong, Do Duy Ngoc
Key Laboratory of Animal Cell Technology, National Institute of Animal Sciences, Thuyphuong, Tuliem, Hanoi 100000, Vietnam.
Biodiversity Conservation and Tropical Diseases Research Institute, Hanoi 100000, Vietnam.
Vet Sci. 2022 Jul 25;9(8):379. doi: 10.3390/vetsci9080379.
Mastitis is one of the most widespread diseases in dairy cows and causes huge losses for the dairy industry. Molecular markers can be used for the quick diagnosis of mastitis infection, consequently reducing the loss caused by this disease. Lactoferrin (LTF) and Toll-like receptor 2 (TLR2) have been suggested as candidate genes for mastitis; however, their associations with the mastitis incidence and milk components have not been reported in Vietnamese Holstein cows. This study examined the association of TLR2 and LTF polymorphisms with subclinical mastitis and milk components in the Holstein breed raised in Vietnam. Among 192 samples, we identified 44 mastitis-positive samples (22.92%). The mastitis significantly reduced the fat and lactose components in milk (p < 0.001) but increased the protein concentration in milk. A total of 94 (49%) and 98 (51%) cows had AA and AB genotypes for the LTF gene, respectively. No significant association was found between the LTF genotypes and the milk component traits or mastitis incidence (p > 0.05). The interaction between LTF and mastitis incidence was significantly associated with the protein percentage (p = 0.01). A total of 78, 76, and 38 cows had genotypes GG, GT, and TT for the TLR2 gene, respectively. TLR2 genotypes were not significantly associated with mastitis incidence (p > 0.05) but were significantly associated with pH value (p = 0.03). The interaction between TLR2 and mastitis incidence was significantly associated with the fat (p = 0.02) and protein percentage (p = 0.04). Further studies are required to confirm the roles of LTF and TFL2 in mastitis in the Holstein breed in Vietnam.
乳腺炎是奶牛中最普遍的疾病之一，给乳制品行业造成巨大损失。分子标记可用于乳腺炎感染的快速诊断，从而减少这种疾病造成的损失。乳铁蛋白（LTF）和Toll样受体2（TLR2）已被认为是乳腺炎的候选基因；然而，在越南荷斯坦奶牛中，它们与乳腺炎发病率和牛奶成分的关联尚未见报道。本研究调查了TLR2和LTF基因多态性与越南饲养的荷斯坦品种亚临床乳腺炎和牛奶成分之间的关联。在192个样本中，我们鉴定出44个乳腺炎阳性样本（22.92%）。乳腺炎显著降低了牛奶中的脂肪和乳糖成分（p < 0.001），但提高了牛奶中的蛋白质浓度。共有94头（49%）和98头（51%）奶牛的LTF基因分别为AA和AB基因型。未发现LTF基因型与牛奶成分性状或乳腺炎发病率之间存在显著关联（p > 0.05）。LTF与乳腺炎发病率之间的相互作用与蛋白质百分比显著相关（p = 0.01）。共有78头、76头和38头奶牛的TLR2基因分别为GG、GT和TT基因型。TLR2基因型与乳腺炎发病率无显著关联（p > 0.05），但与pH值显著相关（p = 0.03）。TLR2与乳腺炎发病率之间的相互作用与脂肪（p = 0.02）和蛋白质百分比（p = 0.04）显著相关。需要进一步研究以证实LTF和TFL2在越南荷斯坦品种乳腺炎中的作用。
