Ph.D.'s Degree Program in Biodiversity and Ethnobiology (International Program), Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
J Helminthol. 2022 Jul 27;96:e52. doi: 10.1017/S0022149X22000463.
The assassin snail genus is widespread in Southeast Asia, and is distributed all over the world via the aquarium trade. One species of genus , , is known to act as intermediate host of parasitic trematodes. This study investigates the taxonomic diversity of larval trematodes infecting . and in Thailand. Larval trematodes were identified by combining morphological and DNA sequence data (cytochrome oxidase I and internal transcribed spacer 2). Species delimitation methods were used to explore larval trematode species boundaries. A total of 1107 specimens of sp. were collected from 25 localities in Thailand. Sixty-two specimens of . ( = 33) and . ( = 29) were infected by zoogonid cercariae, heterophyid metacercariae and echinostome metacercariae, with an overall prevalence of 5.6% (62/1107) and population-level prevalences in the range of 0.0-22.3%. DNA sequence data confirmed that the larval trematodes belong to the families Echinostomatidae, Heterophyidae and Zoogonidae. As such, this is the first report of zoogonid cercariae and heterophyid metacercariae in . , and echinostome metacercariae in . . Moreover, this study provides evidence of tentative species-level differentiation between Thai sp. and Cambodian , as well as within , and .
assassin 属在东南亚广泛分布，并通过水族馆贸易在全球范围内传播。 assassin 属的一种已知是寄生吸虫的中间宿主。本研究调查了感染泰国 assassin 属幼虫吸虫的分类多样性。幼虫吸虫通过结合形态学和 DNA 序列数据（细胞色素氧化酶 I 和内部转录间隔区 2）进行鉴定。使用物种delimitation 方法探索幼虫吸虫的物种边界。从泰国 25 个地点共采集了 1107 个 assassin sp. 标本。62 个 assassin . ( = 33) 和. ( = 29) 被 Zoogonid 尾蚴、异形尾蚴和 Echinostome 尾蚴感染，总感染率为 5.6%（62/1107），种群水平感染率在 0.0-22.3%之间。DNA 序列数据证实，幼虫吸虫属于 Echinostomatidae、Heterophyidae 和 Zoogonidae 科。因此，这是首次在 assassin . 中报告 Zoogonid 尾蚴和异形尾蚴，以及在 assassin . 中报告 Echinostome 尾蚴。此外，本研究还提供了泰国 assassin sp.与柬埔寨 assassin 之间以及 assassin . 、 assassin . 和 assassin . 内部存在暂定种水平分化的证据。
