Parasitology and Medical Malacology Research Unit, Department of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom, 73000, Thailand.
Department of Biodiversity of Animals, Universität Hamburg, and Leibniz Institute for the Analysis of Biodiversity Change (LIB), Zoological Museum, Martin-Luther-King-Platz 3, 20146, Hamburg, Germany.
Parasitol Res. 2024 Oct 1;123(10):338. doi: 10.1007/s00436-024-08345-4.
This study aimed to investigate the occurrence of larval trematode infections in bithyniid snails across five provinces in southern Thailand. A total of 1,413 Bithynia siamensis snails were collected between October 2021 and October 2022 using handpicking and scooping methods. Among these, 844 were identified as B. s. siamensis and 569 as B. s. goniomphalos. The snail samples were examined for parasitic infections in the laboratory using shedding and crushing methods. Among the 27 sampling sites, snails from 6 sites were infected with various trematode species (infection rate: 4.95%, 70/1,413). Evaluation of the morphological and internal organ characteristics of the cercariae allowed for the categorization of the trematodes into five species of trematodes (belonging to four families). The identified species included Echinochasmus pelecani (family: Echinostomatidae), Echinostoma revolutum (family: Echinostomatidae), Haematoloechus similis (family: Haematoloechidae), Loxogenoides bicolor (family Lecithodendriidae), and Stictodora tridactyla (family: Heterophyidae), and the infection rates for these species were 3.26% (46/1,413), 0.35% (5/1,413), 0.42% (6/1,413), 0.78% (11/1,413), and 0.14% (2/1,413), respectively. The cercariae from the identified trematode species were studied for DNA analysis. The phylogenetic lineage reveals relationships among the species, confirming the morphological distinctions.
本研究旨在调查泰国南部五个省份比氏双脐螺中幼虫期吸虫感染的发生情况。2021 年 10 月至 2022 年 10 月期间，使用手工采集和挖取方法共采集了 1413 只比氏双脐螺。其中 844 只鉴定为比氏双脐螺 siamensis，569 只为比氏双脐螺 goniomphalos。在实验室中，使用脱落和压碎法检查螺样是否存在寄生虫感染。在 27 个采样点中，有 6 个采样点的螺感染了各种吸虫（感染率：4.95%，70/1413）。对尾蚴的形态和内部器官特征进行评估，可将吸虫分为 5 种吸虫（属于 4 科）。鉴定出的物种包括棘口吸虫 pelecani（棘口科）、Echinostoma revolutum（棘口科）、相似血居吸虫 Haematoloechus similis（血居科）、双色豆形吸虫 Loxogenoides bicolor（豆形科）和三齿拟腹吸科吸虫 Stictodora tridactyla（拟腹吸科），这些物种的感染率分别为 3.26%（46/1413）、0.35%（5/1413）、0.42%（6/1413）、0.78%（11/1413）和 0.14%（2/1413）。对鉴定出的吸虫的尾蚴进行 DNA 分析。系统发育谱系揭示了这些物种之间的关系，证实了形态学上的区别。
