环状 RNA 0008768 通过 miR-330-3p/PTEN 轴抑制胰腺癌进展。

Circ_0008768 Suppresses the Pancreatic Cancer Progression via miR-330- 3p/PTEN Axis.

机构信息

Department of Hepatobiliary and Pancreatic Surgery, The First Hospital of Shanxi Medical University, Taiyuan 030000, Shanxi, China.

Department of General Surgery, The Sixth Hospital of Shanxi Medical University, Taiyuan 030000, Shanxi, China.

出版信息

Protein Pept Lett. 2022;29(9):796-805. doi: 10.2174/0929866529666220726144619.

DOI:10.2174/0929866529666220726144619

PMID:35894462

Abstract

OBJECTIVE

To probe the role of circular RNA_0008768 (circ_0008768) in the development of pancreatic cancer (PC) and its regulatory mechanism.

METHODS

The expression levels of circ_0008768, miR-330-3p, and PTEN mRNA in PC tissues and cells were detected using qRT-PCR. Cell proliferation, migration and invasion of PC cells were detected by CCK-8 method, EdU method, and Transwell assay. The targeting relationship between circ_0008768 and miR-330-3p, as well as miR-330-3p and PTEN mRNA 3'UTR was analyzed by the dual-luciferase reporter assay. PTEN expression levels in PC cells were analyzed by Western blot.

RESULTS

The expression levels of circ_0008768 and PTEN mRNA were significantly reduced in both PC tissues and cell lines. Overexpression of circ_0008768 exerted an inhibitory effect on the proliferation, migration and invasion of PC cells. Knocking down circ_0008768 showed the opposite effect. Circ_0008768 directly targeted and negatively regulated the expression of miR-330- 3p. PTEN was identified as a downstream target gene of miR-330-3p. Circ_0008768 could positively regulate the expression of PTEN.

CONCLUSION

In PC, circ_0008768 can act as a tumor-suppressive factor to inhibit the development of PC by regulating the miR-330-3p/PTEN molecular axis.

摘要

目的

探讨环状 RNA_0008768（circ_0008768）在胰腺癌（PC）发展中的作用及其调控机制。

方法

采用 qRT-PCR 检测 PC 组织和细胞中 circ_0008768、miR-330-3p 和 PTEN mRNA 的表达水平。采用 CCK-8 法、EdU 法和 Transwell 实验检测 PC 细胞的增殖、迁移和侵袭能力。采用双荧光素酶报告实验分析 circ_0008768 与 miR-330-3p 以及 miR-330-3p 与 PTEN mRNA 3'UTR 的靶向关系。采用 Western blot 分析 PC 细胞中 PTEN 的表达水平。

结果

circ_0008768 和 PTEN mRNA 的表达水平在 PC 组织和细胞系中均明显降低。过表达 circ_0008768 对 PC 细胞的增殖、迁移和侵袭能力具有抑制作用。敲低 circ_0008768 则表现出相反的效果。circ_0008768 可直接靶向并负调控 miR-330-3p 的表达。PTEN 被鉴定为 miR-330-3p 的下游靶基因。circ_0008768 可正向调控 PTEN 的表达。