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通过栒子合成的氧化铜纳米粒子的仿生制备及其在染料去除中的应用:抗氧化和抗菌研究

Bioinspired fabrication of CuONPs synthesized via Cotoneaster and application in dye removal: antioxidant and antibacterial studies.

作者信息

Isiksel Ecem, Attar Azade, Mutlu Ozge, Altikatoglu Yapaoz Melda

机构信息

Department of Chemistry, Faculty of Science and Letters, Yildiz Technical University, Davutpasa Campus, Istanbul, 34220, Turkey.

Department of Bioengineering, Faculty of Chemical & Metallurgical Engineering, Yildiz Technical University, Davutpasa Campus, Istanbul, 34220, Turkey.

出版信息

Environ Sci Pollut Res Int. 2023 Jan;30(1):161-171. doi: 10.1007/s11356-022-22149-y. Epub 2022 Jul 27.

Abstract

In this study, bioinspired fabrication of copper oxide nanoparticles (CuONPs) which are widely researched in nanotechnology field with Cotoneaster extract was performed. Cotoneaster plant extract was chosen as a good antioxidant and antibacterial agent in terms of the amount of phenolic and flavonoid compounds it contains. The obtained CuONPs were characterized by UV-Vis, FTIR, and SEM analyses. Antibacterial activity of the fabricated nanoparticles was evaluated against Escherichia coli and Staphylococcus aureus. Total phenolic compound, total flavonoid amount, and reducing power of the CuONPs were determined. Furthermore, paint removal properties of copper oxide nanoparticles on various dyes were investigated. Fabrication of the CuONPs was evaluated morphologically by color change and in UV spectrum by SPR band at 338 nm. The characteristic peak of CuONPs at 621 cm was monitored employing FT-IR. SEM results showed that the fabricated CuONPs were spherical and between 50 and 160 nm. The CuONPs represented notable antibacterial efficiency against E. coli and S. aureus with inhibition zone of 19 ± 1 and 23 ± 2, respectively employing disk diffusion. The antioxidant properties of the CuONPs were also confirmed. Total phenolic substance content of the CuONP solution was 6.04 μg pyrocathecol equivalent/mg nanoparticle and total flavonoid content value was found as 122.46 μg catechin equivalent/mg nanoparticle. The reducing power of the fabricated CuONPs was found to be good when compared to the standard antioxidants BHA and α-tocopherol. In addition, the decolorization efficiency of the fabricated CuONPs has a strong potential on the industrial dye removal of neutral red and naphthol blue black.

摘要

在本研究中,利用枸子属植物提取物对纳米技术领域广泛研究的氧化铜纳米颗粒(CuONPs)进行了仿生制备。枸子属植物提取物因其所含酚类和黄酮类化合物的量而被选为优良的抗氧化剂和抗菌剂。通过紫外可见光谱(UV-Vis)、傅里叶变换红外光谱(FTIR)和扫描电子显微镜(SEM)分析对所得的CuONPs进行了表征。评估了制备的纳米颗粒对大肠杆菌和金黄色葡萄球菌的抗菌活性。测定了CuONPs的总酚类化合物、总黄酮含量和还原能力。此外,还研究了氧化铜纳米颗粒对各种染料的脱色性能。通过颜色变化从形态学上评估了CuONPs的制备,并通过338 nm处的表面等离子体共振(SPR)带在紫外光谱中进行了评估。利用傅里叶变换红外光谱监测了CuONPs在621 cm处的特征峰。扫描电子显微镜结果表明,制备的CuONPs呈球形,粒径在50至160 nm之间。采用纸片扩散法,CuONPs对大肠杆菌和金黄色葡萄球菌表现出显著的抗菌效率,抑菌圈分别为19±1和23±2。CuONPs的抗氧化性能也得到了证实。CuONP溶液的总酚类物质含量为6.04 μg邻苯二酚当量/mg纳米颗粒,总黄酮含量值为122.46 μg儿茶素当量/mg纳米颗粒。与标准抗氧化剂丁基羟基茴香醚(BHA)和α-生育酚相比,制备的CuONPs的还原能力良好。此外,制备的CuONPs对工业染料中性红和萘酚蓝黑的脱色效率具有很强的潜力。

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