功能化绿色合成氧化铜纳米粒子：作为新型抗菌和抗生物膜剂的前景。
Functionalized Green Synthesis of CuO-Nanoparticles: Novel Prospects as Antibacterial and Antibiofilm Agents.
机构信息
Faculty of Agricultural Sciences, Department of Agricultural Microbiology, Aligarh Muslim University, Uttar Pradesh, Aligarh 202002, India.
Zoology Department, College of Sciences, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
出版信息
Biomolecules. 2020 Jan 22;10(2):169. doi: 10.3390/biom10020169.
Chemically synthesized copper oxide nanoparticles (CuONPs) involve the generation of toxic products, which narrowed its biological application. Hence, we have developed a one-pot, green method for CuONP production employing the leaf extract of (CLE). Gas chromatography-mass spectrometry (GC-MS) analysis confirmed the capping of CuONPs by CLE esters (CLE-CuONPs). Fourier-transform infrared (FTIR) showed phenolics, sugars, and proteins mediated nucleation and stability of CLE-CuONPs. X-ray diffraction (XRD) and transmission electron microscopy (TEM) revealed CLE-CuONPs between 11.4 to 14.5 nm. -1 (MRSA-1), 2 (MSSA-2) exposed to CLE-CuONPs (1500 µg/mL) showed 51.4%, 32.41% survival, while 336 (336) exposed to 1000 µg/mL CLE-CuONPs showed 45.27% survival. Scanning electron microscopy (SEM) of CLE-CuONPs treated -336, MSSA-2 and MRSA-1 showed morphological deformations. The biofilm production by -336 and MRSA-1 also declined to 33.0 ± 3.2% and 49.0 ± 3.1% at 2000 µg/mL of CLE-CuONPs. Atomic absorption spectroscopy (AAS) showed 22.80 ± 2.6%, 19.2 ± 4.2%, and 16.2 ± 3.6% accumulation of Cu in -336, MSSA-2, and MRSA-1. Overall, the data exhibited excellent antibacterial and antibiofilm efficacies of esters functionalized CLE-CuONPs, indicating its putative application as a novel nano-antibiotic against multi drug resistance (MDR) pathogenic clinical isolates.
化学合成的氧化铜纳米粒子 (CuONPs) 会产生有毒产物，限制了其生物应用。因此，我们开发了一种使用 （CLE）叶提取物的一锅、绿色方法来生产 CuONPs。气相色谱-质谱联用 (GC-MS) 分析证实了 CuONPs 被 CLE 酯（CLE-CuONPs）封端。傅里叶变换红外 (FTIR) 显示了酚类、糖和蛋白质介导的 CLE-CuONPs 的成核和稳定性。X 射线衍射 (XRD) 和透射电子显微镜 (TEM) 显示 CLE-CuONPs 的粒径在 11.4 到 14.5nm 之间。-1 (MRSA-1)、2 (MSSA-2) 暴露于 CLE-CuONPs（1500µg/mL）时，存活率分别为 51.4%和 32.41%，而 336（336）暴露于 1000µg/mL CLE-CuONPs 时，存活率为 45.27%。暴露于 CLE-CuONPs 的-336、MSSA-2 和 MRSA-1 的扫描电子显微镜 (SEM) 显示出形态变形。-336 和 MRSA-1 的生物膜生成也分别下降至 2000µg/mL CLE-CuONPs 时的 33.0±3.2%和 49.0±3.1%。原子吸收光谱 (AAS) 显示-336、MSSA-2 和 MRSA-1 中 Cu 的积累量分别为 22.80±2.6%、19.2±4.2%和 16.2±3.6%。总的来说，数据显示出酯功能化 CLE-CuONPs 的优异抗菌和抗生物膜功效，表明其作为一种新型纳米抗生素在针对多药耐药 (MDR) 临床分离株方面具有潜在应用。
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