United States Department of Agriculture, Agricultural Research Service, Appalachian Fruit Research Station (USDA-ARS-AFRS), Kearneysville, WV, USA.
Electrical Engineering Department, Universidad Tecnológica de Pereira, Pereira, Colombia.
Methods Mol Biol. 2022;2539:87-94. doi: 10.1007/978-1-0716-2537-8_10.
Images are used frequently in plant phenotyping to capture measurements. This chapter offers a repeatable method for capturing two-dimensional measurements of plant parts in field or laboratory settings using a variety of camera styles (cellular phone, DSLR), with the addition of a printed calibration pattern. The method is based on calibrating the camera using information available from the EXIF tags from the image, as well as visual information from the pattern. Code is provided to implement the method, as well as a dataset for testing. We include steps to verify protocol correctness by imaging an artifact. The use of this protocol for two-dimensional plant phenotyping will allow data capture from different cameras and environments, with comparison on the same physical scale. We abbreviate this method as CASS, CAmera aS Scanner.
图像在植物表型中被频繁用于捕获测量值。本章提供了一种可重复的方法，用于使用各种相机类型（手机、数码单反相机）在野外或实验室环境中捕获植物部分的二维测量值，并添加了打印的校准图案。该方法基于使用图像的 EXIF 标签中的可用信息以及图案的视觉信息来校准相机。我们提供了实现该方法的代码以及一个用于测试的数据集。我们还包括了通过对人工制品成像来验证协议正确性的步骤。该协议用于二维植物表型学，可以允许使用不同的相机和环境进行数据捕获，并在相同的物理尺度上进行比较。我们将这种方法简称为 CASS，即相机作为扫描仪。
