Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada.
Agassiz Research and Development Centre, Agriculture and Agri-Food Canada, British Columbia, V0M 1A0, Agassiz, Canada.
J Chem Ecol. 2022 Jun;48(5-6):491-501. doi: 10.1007/s10886-022-01367-1. Epub 2022 Jul 27.
Synthetic sex pheromone lures are useful tools to monitor and control populations of adult click beetles (Coleoptera: Elateridae). However, sex pheromones for Agriotes click beetle species native to North America have yet to be identified. Here we report the identification and field testing of the sex pheromone of Agriotes ferrugineipennis. Headspace volatiles from female beetles were collected on Porapak Q, and aliquots of Porapak extract were analyzed by gas chromatographic-electroantennographic detection (GC-EAD) and GC-mass spectrometry. 7-Methyloctyl 7-methyloctanoate (7Me7Me) emitted by females was more abundant and elicited much stronger responses from male antennae than the aldehydes octanal and nonanal and the ketone 6,10,14-trimethyl-2-pentadecanone. In a field experiment, captures of A. ferrugineipennis males in traps baited with candidate pheromone components exceeded those of unbaited control traps, on average by nearly 1,200 times. Neither the ketone nor the aldehydes as lure constituents appeared to alter captures of males in 7Me7Me-baited traps. We conclude that 7Me7Me is the major, and possibly the only, sex attractant pheromone component of female A. ferrugineipennis.
合成性信息素诱饵是监测和控制成虫叩头虫（鞘翅目：叩甲科）种群的有用工具。然而，尚未鉴定出原产于北美的 Agriotes 叩甲物种的性信息素。在这里，我们报告了 Agriotes ferrugineipennis 的性信息素的鉴定和田间测试。雌性甲虫的头空间挥发物用 Porapak Q 收集，并用 Porapak 提取物的等分试样进行气相色谱-触角电位检测（GC-EAD）和 GC-质谱分析。与醛类辛醛和壬醛以及酮类 6,10,14-三甲基-2-十五烷酮相比，雌性释放的 7-甲基辛基 7-甲基辛酸酯（7Me7Me）含量更丰富，对雄性触角的反应也更强。在田间试验中，用候选信息素成分诱捕的 A. ferrugineipennis 雄性捕获量超过了未诱捕对照诱捕器的捕获量，平均高出近 1200 倍。作为引诱剂成分的酮和醛都没有改变 7Me7Me 诱捕器中雄性的捕获量。我们得出结论，7Me7Me 是雌性 A. ferrugineipennis 的主要（可能是唯一的）性信息素引诱剂成分。
