SARS-CoV-2 联合诊断：快速抗原检测作为核酸检测的辅助手段。
Combined Diagnosis of SARS-CoV-2: Rapid Antigen Detection as an Adjunct to Nucleic Acid Detection.
机构信息
Department of Laboratory Medicine, First Hospital of Jilin University, Changchun, China.
Bachelor of Biomedicine, University of Melbourne, Melbourne, Victoria, Australia.
出版信息
Lab Med. 2023 Mar 7;54(2):e37-e43. doi: 10.1093/labmed/lmac089.
Coronavirus disease 2019 is a serious threat to human life, and early diagnosis and screening can help control the COVID-19 pandemic. The high sensitivity of reverse transcriptase-polymerase chain reaction (RT-PCR) assay is the gold standard for the diagnosis of COVID-19, but there are still some false-negative results. Rapid antigen detection (RAD) is recommended by the World Health Organization (WHO) as a screening method for COVID-19. This review analyzed the characteristics of RDT and found that although the overall sensitivity of RAD was not as high as that of RT-PCR, but RAD was more sensitive in COVID-19 patients within 5 days of the onset of symptoms and in COVID-19 patients with Ct ≤ 25. Therefore, RAD can be used as an adjunct to RT-PCR for screening patients with early COVID-19. Finally, this review provides a combined diagnostic protocol for RAD and nucleic acid testing with the aim of providing a feasible approach for COVID-19 screening.
新型冠状病毒肺炎是对人类生命的严重威胁，早期诊断和筛查有助于控制 COVID-19 大流行。逆转录-聚合酶链反应（RT-PCR）检测的高灵敏度是 COVID-19 诊断的金标准，但仍存在一些假阴性结果。世界卫生组织（WHO）推荐快速抗原检测（RAD）作为 COVID-19 的筛查方法。本综述分析了 RDT 的特点，发现虽然 RAD 的总体灵敏度不如 RT-PCR 高，但在症状出现后 5 天内的 COVID-19 患者和 Ct 值≤25 的 COVID-19 患者中，RAD 的灵敏度更高。因此，RAD 可作为 RT-PCR 的辅助手段用于筛查早期 COVID-19 患者。最后，本综述提出了 RAD 和核酸检测的联合诊断方案，旨在为 COVID-19 筛查提供一种可行的方法。
Zotero 插件