Halo Marko, Tirpák Filip, Massányi Martin, Kováč Ján, Mlyneková Eva, Greń Agnieszka, Halo Marko, Massányi Peter
1 AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic.
2 Institute of Applied Biology, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic.
Acta Vet Hung. 2022 Jun 16. doi: 10.1556/004.2022.00016.
The purpose of this study was to evaluate the dose- and time-dependent effect of caffeine treatment on the motility and viability of stallion spermatozoa at different temperatures. Six dose groups (A to F) were established with changing caffeine concentrations (from 0.625 to 10 mg/mL). The control samples were prepared by diluting the ejaculate only with physiological salt solution. The samples were examined after 0, 1, 2 and 3 h of incubation at 5 °C and 37 °C. The motility parameters were evaluated by Computer Assisted Semen Analyzer (CASA) system, and the viability was assessed by the mitochondrial toxicity test at the end of the incubation. A positive effect of the lowest tested caffeine concentration on the motility parameters was observed throughout the incubation period at 5 °C. At the end of the 3h incubation, the viability in every sample in these groups, treated with any caffeine concentration, showed lower values compared to the control. At the higher incubation temperature (37 °C), caffeine positively affected the motility in samples B (P < 0.05) and D, E, F (P < 0.001) after 3 h of incubation; however, the viability showed a slightly decreasing tendency. Our results suggest that caffeine, in an optimal concentration, may be used as a component of stallion semen extenders.
本研究的目的是评估咖啡因处理对不同温度下马精子活力和生存力的剂量和时间依赖性影响。通过改变咖啡因浓度（从0.625至10mg/mL）建立了六个剂量组（A至F）。对照样品仅用生理盐溶液稀释精液制备。样品在5℃和37℃孵育0、1、2和3小时后进行检查。通过计算机辅助精液分析仪（CASA）系统评估活力参数，并在孵育结束时通过线粒体毒性试验评估生存力。在5℃的整个孵育期内，观察到所测试的最低咖啡因浓度对活力参数有积极影响。在孵育3小时结束时，与对照组相比，这些组中用任何咖啡因浓度处理的每个样品的生存力均显示较低值。在较高的孵育温度（37℃）下，孵育3小时后，咖啡因对样品B（P<0.05）和D、E、F（P<0.001）的活力有积极影响；然而，生存力呈略有下降的趋势。我们的结果表明，最佳浓度的咖啡因可作为马精液稀释剂的一种成分。
