Am J Vet Res. 2022 Jul 13;83(8):ajvr.21.10.0172. doi: 10.2460/ajvr.21.10.0172.
To investigate the clinical value of selected biomarkers for evaluation of systemic inflammatory response and pulmonary inflammation and damage pattern in calves with different pneumonia forms.
16 calves with fibrinous pneumonia (FP group; infected with Mannheimia haemolytica or Pasteurella multocida), 12 calves with caseonecrotic pneumonia (CNP group; infected with Mycoplasma bovis), and 10 healthy calves (C group) based on results of bronchoalveolar lavage fluid (BALF) examination.
Blood and BALF samples were collected. Annexin-A2 (Anx-A2), endothelin-1 (ET-1), calgranulin B (S100A9), transforming growth factor-β1 (TGF-β1), tumor necrosis factor-α (TNF-α), interleukin-17A (IL-17A), haptoglobin (Hp), lipopolysaccharide-binding protein (LBP), and albumin (Alb) concentrations and lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) activities were measured and compared across groups.
Serum concentrations of Anx-A2, S100A9, TGF-β1, TNF-α, IL-17A, Hp, and LBP and activities of LDH and ALP were higher and Alb concentrations were lower for the pneumonia groups versus C group. BALF concentration ratios of S100A9:Alb, LBP:Alb, LDH:Alb, and ALP:Alb were higher for the pneumonia groups versus C group (P < .05). BALF concentration ratios of Anx-A2:Alb, TGF-β1:Alb, and IL-17A:Alb were higher for the FP group versus other groups (P < .05).
Results indicated that serum Anx-A2, S100A9, TGF-β1, TNF-α, IL-17A, Hp, LBP, and Alb concentrations were useful in determining the systemic inflammatory response and that BALF concentration ratios of S100A9:Alb, TGF-β1:Alb, LBP:Alb, and ALP:Alb were useful biomarkers in determining pulmonary inflammation and damage. Measurements of BALF concentration ratios of Anx-A2:Alb, TGF-β1:Alb, and IL-17A:Alb could be beneficial to defining fibrinous characterization of pulmonary inflammation.
探讨选择生物标志物评估不同肺炎形式犊牛全身炎症反应和肺炎症及损伤模式的临床价值。
16 头纤维蛋白性肺炎（FP 组；感染溶血性曼海姆菌或多杀性巴氏杆菌）、12 头坏死性肺炎（CNP 组；感染牛支原体）和 10 头健康犊牛（C 组）基于支气管肺泡灌洗液（BALF）检查结果。
采集血液和 BALF 样本。比较各组间膜联蛋白-A2（Anx-A2）、内皮素-1（ET-1）、钙粒蛋白 B（S100A9）、转化生长因子-β1（TGF-β1）、肿瘤坏死因子-α（TNF-α）、白细胞介素-17A（IL-17A）、触珠蛋白（Hp）、脂多糖结合蛋白（LBP）和白蛋白（Alb）浓度以及乳酸脱氢酶（LDH）和碱性磷酸酶（ALP）活性。
与 C 组相比，肺炎组血清 Anx-A2、S100A9、TGF-β1、TNF-α、IL-17A、Hp 和 LBP 浓度和 LDH 和 ALP 活性更高，Alb 浓度更低。与 C 组相比，肺炎组 BALF 中 S100A9:Alb、LBP:Alb、LDH:Alb 和 ALP:Alb 浓度比值更高（P<0.05）。与其他组相比，FP 组 BALF 中 Anx-A2:Alb、TGF-β1:Alb 和 IL-17A:Alb 浓度比值更高（P<0.05）。
结果表明，血清 Anx-A2、S100A9、TGF-β1、TNF-α、IL-17A、Hp、LBP 和 Alb 浓度有助于确定全身炎症反应，BALF 中 S100A9:Alb、TGF-β1:Alb、LBP:Alb 和 ALP:Alb 浓度比值有助于确定肺炎症和损伤。BALF 中 Anx-A2:Alb、TGF-β1:Alb 和 IL-17A:Alb 浓度比值的测量可能有助于确定肺炎症的纤维蛋白特征。
