Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, USA.
Division of Biochemical Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, USA.
J Environ Sci Health C Toxicol Carcinog. 2022;40(1):106-118. doi: 10.1080/26896583.2022.2044242. Epub 2022 Mar 11.
In vitro genotoxicity testing plays an important role in chemical risk assessment. The human B-lymphoblastoid cell line TK6 is widely used as a standard cell line for regulatory safety evaluations. Like many other mammalian cell lines, TK6 cells have limited metabolic capacity; therefore, usually require a source of exogenous metabolic activation for use in genotoxicity testing. Previously, we developed a set of TK6-derived cell lines that individually express one of fourteen cytochrome P450s (CYPs). In the present study, we surveyed a panel of major Phase II drug-metabolizing enzymes to characterize their baseline expression in TK6 cells. These results may serve as a reference enzymatic profile of this commonly used cell line.
体外遗传毒性测试在化学风险评估中发挥着重要作用。人 B 淋巴细胞白血病细胞系 TK6 被广泛用作监管安全评估的标准细胞系。与许多其他哺乳动物细胞系一样，TK6 细胞的代谢能力有限；因此，通常需要外源代谢活化源才能用于遗传毒性测试。以前，我们开发了一组单独表达十四种细胞色素 P450（CYPs）之一的 TK6 衍生细胞系。在本研究中，我们调查了一组主要的 II 相药物代谢酶，以表征它们在 TK6 细胞中的基础表达。这些结果可以作为该常用细胞系的参考酶谱。
