Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea.
Nano Lett. 2022 Aug 10;22(15):6121-6127. doi: 10.1021/acs.nanolett.2c01287. Epub 2022 Jul 27.
DNA-templated copper nanoclusters (CuNCs) have limited applications because of their low fluorescence stability (several tens of minutes). In this study, we prepared CuNCs with improved temporal fluorescence stability by introducing fructose into the CuNC synthesis process and optimizing the reaction conditions. The inclusion of fructose increased the operating lifetime of CuNCs by approximately 5200-fold from 30 min to 108 days and improved their stability against heat, acids, and bases compared to CuNCs synthesized under original conditions. In addition, the fluorescence signal of CuNCs was maintained for a significantly longer time when stored at refrigeration (4 °C) and freezing (-20 °C) temperatures. Importantly, this method did not require the addition of substances other than fructose or any additional physicochemical treatment to maintain the fluorescence of DNA-templated CuNCs for more than several tens of days. As such, this study could serve as a basis to improve the stability of CuNCs for various applications.
DNA 模板铜纳米团簇 (CuNCs) 的荧光稳定性有限（几十分钟），因此应用受到限制。在这项研究中，我们通过在 CuNCs 的合成过程中引入果糖并优化反应条件，制备出了具有改进的时间荧光稳定性的 CuNCs。与原始条件下合成的 CuNCs 相比，果糖的加入使 CuNCs 的工作寿命从 30 分钟延长了约 5200 倍，达到 108 天，并且提高了其对热、酸和碱的稳定性。此外，CuNCs 的荧光信号在冷藏（4°C）和冷冻（-20°C）温度下储存时，保持更长的时间。重要的是，这种方法除了果糖之外不需要添加其他物质，也不需要进行任何额外的物理化学处理，就可以使 DNA 模板铜纳米团簇的荧光持续数十天以上。因此，这项研究可以为提高 CuNCs 的稳定性，以应用于各种领域提供基础。
