Xue Yijiong, Bao Chengqi, Liu Hui, Ma Fanghui, Yang Minghui, Li Xiaoqing
Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Xia Duopu Health Center of Ningxiang, Changsha 410605, China.
Biosensors (Basel). 2025 Jan 15;15(1):49. doi: 10.3390/bios15010049.
A fluorescence probe for "switch-on" detection of alkaline phosphatase (ALP) was developed based on Au nanoclusters anchored MnO nanosheets (Au NCs-MnO NSs), which were synthesized using bovine serum albumin (BSA) as template through a simple one-pot approach. In the sensing system, MnO NSs function as both energy acceptors and target identifiers, effectively quenches the fluorescence of Au NCs via fluorescence resonance energy transfer (FRET). The presence of ALP catalyzes the hydrolysis of L-ascorbic acid-2-phosphate (AAP) to ascorbic acid (AA), reducing MnO NSs to Mn and facilitate the fluorescence recovery of Au NCs. The fluorescence assay offers the advantages of facile preparation, cost-effectiveness, good specificity, and high sensitivity. Moreover, the assay exhibits a broad linear range (0.005 U/mL to 8 U/mL) for ALP detection with a remarkable limit of detection of 0.0015 U/mL. Notably, this assay demonstrates promising applicability for detection ALP in human serum samples, thereby providing valuable potential for clinical applications.
基于以牛血清白蛋白（BSA）为模板通过简单的一锅法合成的金纳米簇锚定的MnO纳米片（Au NCs-MnO NSs），开发了一种用于“开启”检测碱性磷酸酶（ALP）的荧光探针。在传感系统中，MnO NSs既作为能量受体又作为目标识别物，通过荧光共振能量转移（FRET）有效地淬灭Au NCs的荧光。ALP的存在催化L-抗坏血酸-2-磷酸酯（AAP）水解为抗坏血酸（AA），将MnO NSs还原为Mn并促进Au NCs的荧光恢复。该荧光测定法具有制备简便、成本效益高、特异性好和灵敏度高的优点。此外，该测定法对ALP检测具有宽线性范围（0.005 U/mL至8 U/mL），检测限低至0.0015 U/mL。值得注意的是，该测定法在检测人血清样品中的ALP方面显示出有前景的适用性，从而为临床应用提供了有价值的潜力。
