College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Institute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot, 010010, China.
Sci Rep. 2022 Jul 27;12(1):12840. doi: 10.1038/s41598-022-17196-x.
Potato is one of the most important staple crops in the world. China is one of the leading producers of potatoes, but the industry faces soilborne diseases such as Verticillium wilt. Most potato planting areas in China rotate the crop with sunflower which is also highly susceptible to Verticillium wilt. The comparison of the biological characteristics and pathogenicity of different mating types of Verticillium dahliae isolated from potato and sunflower in the major planting regions in China is of great importance. This is to help unravel the diversity in V. dahliae population and the sudden increase in infected fields. The diseased samples collected were cultured on PDA and the growing colony of pathogen isolated. Molecular techniques using specific primers were used to identify the V. dahliae pathogens and their mating type of the isolates obtained from the diseased sunflower and potato plants as well as their planting materials. The data obtained revealed that the dominant mating type population in sunflower was MAT1-1, whiles that of potato was MAT1-2, but Race 2 was the only race type identified for all the samples. There was a significant presence of MAT1-1 isolates present in potatoes, which is a new trend. Conventional crop rotation farming using sunflower is causing an increasing prevalence of MAT1-1 and mating type shift of isolates in potato in these regions.
土豆是世界上最重要的主食作物之一。中国是土豆的主要生产国之一，但该行业面临着土传病害，如黄萎病。中国大部分土豆种植区与向日葵轮作，而向日葵也极易感染黄萎病。比较来自中国主要种植区的土豆和向日葵上分离的不同交配型的大丽轮枝菌的生物学特性和致病性具有重要意义。这有助于揭示大丽轮枝菌种群的多样性和感染田块的突然增加。采集的病样在 PDA 上培养，分离出病原菌的生长菌落。使用特异性引物的分子技术用于鉴定从患病向日葵和土豆植株及其种植材料中获得的大丽轮枝菌病原体及其交配型。获得的数据表明，向日葵的优势交配型群体是 MAT1-1，而土豆的是 MAT1-2，但所有样本的唯一鉴定的菌系类型是 Race 2。MAT1-1 分离株在土豆中的存在显著增加，这是一个新趋势。在这些地区，使用向日葵进行常规的作物轮作种植导致 MAT1-1 的流行率增加，以及土豆中分离株的交配型转变。
