Li Gengyun, Mo Yunrong, Lv Junheng, Han Shu, Fan Wei, Zhou Ying, Yang Zhengan, Deng Minghua, Xu Bin, Wang Yanyan, Zhao Kai
Key Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, Yunnan, China.
College of Food Science and Technology, Yunnan Agricultural University, Kunming, China.
Front Plant Sci. 2024 May 28;15:1378748. doi: 10.3389/fpls.2024.1378748. eCollection 2024.
Verticillium wilt, caused by , is a soil-borne disease affecting eggplant. Wild eggplant, recognized as an excellent disease-resistant resource against verticillium wilt, plays a pivotal role in grafting and breeding for disease resistance. However, the underlying resistance mechanisms of wild eggplant remain poorly understood. This study compared two wild eggplant varieties, LC-2 (high resistance) and LC-7 (sensitive) at the phenotypic, transcriptomic, and metabolomic levels to determine the molecular basis of their resistance to verticillium wilt. These two varieties exhibit substantial phenotypic differences in petal color, leaf spines, and fruit traits. Following inoculation with , LC-2 demonstrated significantly higher activities of polyphenol oxidase, superoxide dismutase, peroxidase, phenylalanine ammonia lyase, β-1,3 glucanase, and chitinase than did LC-7. RNA sequencing revealed 4,017 differentially expressed genes (DEGs), with a significant portion implicated in processes associated with disease resistance and growth. These processes encompassed defense responses, cell wall biogenesis, developmental processes, and biosynthesis of spermidine, cinnamic acid, and cutin. A gene co-expression analysis identified 13 transcription factors as hub genes in modules related to plant defense response. Some genes exhibited distinct expression patterns between LC-2 and LC-7, suggesting their crucial roles in responding to infection. Further, metabolome analysis identified 549 differentially accumulated metabolites (DAMs) between LC-2 and LC-7, primarily consisting of compounds such as flavonoids, phenolic acids, lipids, and other metabolites. Integrated transcriptome and metabolome analyses revealed the association of 35 gene-metabolite pairs in modules related to the plant defense response, highlighting the interconnected processes underlying the plant defense response. These findings characterize the molecular basis of LC-2 resistance to verticillium wilt and thus have potential value for future breeding of wilt-resistant eggplant varieties.
黄萎病是由[病原体名称未给出]引起的一种影响茄子的土传病害。野生茄子被认为是抗黄萎病的优良抗病资源，在抗病嫁接和育种中发挥着关键作用。然而，野生茄子潜在的抗性机制仍知之甚少。本研究在表型、转录组和代谢组水平上比较了两个野生茄子品种LC-2（高抗）和LC-7（敏感），以确定它们对黄萎病抗性的分子基础。这两个品种在花瓣颜色、叶刺和果实性状上表现出显著的表型差异。接种[病原体名称未给出]后，LC-2的多酚氧化酶、超氧化物歧化酶、过氧化物酶、苯丙氨酸解氨酶、β-1,3-葡聚糖酶和几丁质酶活性显著高于LC-7。RNA测序揭示了4017个差异表达基因（DEG），其中很大一部分与抗病和生长相关的过程有关。这些过程包括防御反应、细胞壁生物合成、发育过程以及亚精胺、肉桂酸和角质的生物合成。基因共表达分析确定了13个转录因子为与植物防御反应相关模块中的枢纽基因。一些基因在LC-2和LC-7之间表现出不同的表达模式，表明它们在应对感染中起关键作用。此外，代谢组分析确定了LC-2和LC-7之间549个差异积累代谢物（DAM），主要由黄酮类化合物、酚酸、脂质和其他代谢物等化合物组成。转录组和代谢组的综合分析揭示了与植物防御反应相关模块中35个基因-代谢物对的关联，突出了植物防御反应背后相互关联的过程。这些发现表征了LC-2对黄萎病抗性的分子基础，因此对未来抗黄萎病茄子品种的育种具有潜在价值。
