College of Environmental Sciences and Engineering, Peking University; Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing, 100871, People's Republic of China.
State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems, Beijing, 100871, People's Republic of China.
Microbiome. 2022 Jul 28;10(1):111. doi: 10.1186/s40168-022-01294-z.
Antibiotic resistome has been found to strongly interact with the core microbiota in the human gut, yet little is known about how antibiotic resistance genes (ARGs) correlate with certain microbes in large rivers that are regarded as "terrestrial gut."
By creating the integral pattern for ARGs and antibiotic-resistant microbes in water and sediment along a 4300-km continuum of the Yangtze River, we found that human pathogen bacteria (HPB) share 13.4% and 5.9% of the ARG hosts in water and sediment but contribute 64% and 46% to the total number of planktonic and sedimentary ARGs, respectively. Moreover, the planktonic HPB harbored 79 ARG combinations that are dominated by "natural" supercarriers (e.g., Rheinheimera texasensis and Noviherbaspirillum sp. Root189) in river basins.
We confirmed that terrestrial HPB are the major ARG hosts in the river, rather than conventional supercarriers (e.g., Enterococcus spp. and other fecal indicator bacteria) that prevail in the human gut. The discovery of HPB as natural supercarriers in a world's large river not only interprets the inconsistency between the spatial dissimilarities in ARGs and their hosts, but also highlights the top priority of controlling terrestrial HPB in the future ARG-related risk management of riverine ecosystems globally. Video Abstract.
已发现抗生素耐药组与人类肠道中的核心微生物群强烈相互作用，但对于被视为“陆地肠道”的大型河流中抗生素抗性基因（ARGs）与某些微生物之间的相关性知之甚少。
通过在长江 4300 公里连续体上的水和沉积物中创建 ARGs 和抗微生物的整体模式，我们发现人类病原体细菌（HPB）在水和沉积物中共占 ARG 宿主的 13.4%和 5.9%，但分别占浮游和沉积物中总 ARG 的 64%和 46%。此外，浮游 HPB 含有 79 种 ARG 组合，这些组合主要由河流盆地中的“天然”超级载体（例如 Rheinheimera texasensis 和 Noviherbaspirillum sp. Root189）主导。
我们证实，陆地 HPB 是河流中的主要 ARG 宿主，而不是在人类肠道中占主导地位的常规超级载体（例如肠球菌属和其他粪便指示菌）。在世界大河中发现 HPB 是天然超级载体，不仅解释了 ARGs 及其宿主之间空间差异的不一致性，而且还强调了在全球河流生态系统未来的 ARG 相关风险管理中控制陆地 HPB 的首要任务。视频摘要。
