Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
School of Water Resources and Environment, China University of Geosciences (Beijing), Beijing 100083, China.
Ecotoxicol Environ Saf. 2023 Mar 1;252:114603. doi: 10.1016/j.ecoenv.2023.114603. Epub 2023 Feb 2.
The distribution of antibiotic resistance genes (ARGs) in water sources potentially threatens drinking water safety. However, the sources of antibiotic resistome in groundwater are still under-investigated. Here, we evaluated the profiles of antibiotic resistome in peri-urban groundwater and its associated water sources (river and mountain spring) to characterize the antibiotic resistome from natural water sources on groundwater resistome. A total of 261 antibiotic resistome were detected in groundwater, mountain spring, and river samples. The relative abundances of ARGs and mobile genetic elements (MGEs) were significantly higher in the river samples than in spring water and groundwater samples. The resistome profiles were similar between groundwater and spring water but differed from the river samples. According to source tracking results, the groundwater resistome was likely to be derived from springs (28.0%-50.0%) and rivers (28.6%-48.6%), which share the same trend for the source tracking of bacterial communities. Bacterial α-diversity, bacterial β-diversity, and MGEs directly or indirectly affected the ARGs in groundwater samples. Although the abundance of groundwater resistome was not elevated by river and spring water, groundwater resistomes were diverse and may be derived from both river and spring water. We highlight the importance of groundwater resistome and its association with potential water sources, providing a better understanding and basis for the effective control of the ARG proliferation and dissemination in groundwater from exogenous water bodies in the future.
抗生素耐药基因（ARGs）在水源中的分布可能对饮用水安全构成威胁。然而，地下水抗生素耐药组的来源仍未得到充分研究。在这里，我们评估了城市周边地下水中抗生素耐药组的特征，并对其相关水源（河流和山泉）进行了评估，以从天然水源的角度描述地下水耐药组的抗生素耐药组。在地下水、山泉和河流水样中共检测到 261 种抗生素耐药组。河流样本中 ARGs 和移动遗传元件（MGEs）的相对丰度明显高于泉水和地下水样本。地下水和泉水的耐药组谱相似，但与河流样本不同。根据来源追踪结果，地下水耐药组可能来源于泉水（28.0%-50.0%）和河流（28.6%-48.6%），这与细菌群落的来源追踪趋势相同。细菌α多样性、细菌β多样性和 MGEs 直接或间接影响了地下水样本中的 ARGs。尽管河流和泉水并未增加地下水耐药组的丰度，但地下水耐药组具有多样性，可能来自河流和泉水。我们强调了地下水耐药组及其与潜在水源的关联的重要性，为未来从外源水体有效控制地下水 ARG 的增殖和传播提供了更好的理解和基础。
