Maleeva Ksenia A, Kaliya Ilia E, Tkach Anton P, Babaev Anton A, Baranov Michail A, Berwick Kevin, Perova Tatiana S, Baranov Alexander V, Bogdanov Kirill V
Center of Information Optical Technologies, ITMO University, 197101 Saint Petersburg, Russia.
School of Electrical and Electronic Engineering, Technological University Dublin, D07 EWV4 Dublin, Ireland.
Materials (Basel). 2022 Jul 27;15(15):5197. doi: 10.3390/ma15155197.
Surface-enhanced Raman spectroscopy (SERS) is regarded as a versatile tool for studying the composition and structure of matter. This work has studied the preparation of a SERS substrate based on a self-assembling plasmonic nanoparticle film (SPF) in a polymer matrix. Several synthesis parameters for the SPF are investigated, including the size of the particles making up the film and the concentration and type of the self-assembling agent. The result of testing systems with different characteristics is discussed using a model substance (pseudoisocyanin iodide). These models can be useful in the study of biology and chemistry. Research results contain the optimal parameters for SPF synthesis, maximizing the SERS signal. The optimal procedure for SPF assembly is determined and used for the synthesis of composite SPFs within different polymer matrices. SPF in a polymer matrix is necessary for the routine use of the SERS substrate for various types of analytes, including solid samples or those sensitive to contamination. Polystyrene, polyvinyl alcohol (PVA), and polyethylene are investigated to obtain a polymer matrix for SPF, and various methods of incorporating SPF into a polymer matrix are being explored. It is found that films with the best signal enhancement and reproducibility were obtained in polystyrene. The minimum detectable concentration for the SERS substrate obtained is equal to 10 M. We prepared a SERS substrate with an analytical enhancement factor of 2.7 × 10, allowing an increase in the detection sensitivity of analyte solutions of five orders of magnitude.
表面增强拉曼光谱（SERS）被视为研究物质组成和结构的通用工具。这项工作研究了基于聚合物基质中自组装等离子体纳米颗粒薄膜（SPF）的SERS基底的制备。研究了SPF的几个合成参数，包括构成薄膜的颗粒尺寸、自组装剂的浓度和类型。使用模型物质（碘化假异氰蓝）讨论了具有不同特性的测试系统的结果。这些模型在生物学和化学研究中可能有用。研究结果包含了SPF合成的最佳参数，可使SERS信号最大化。确定了SPF组装的最佳程序，并将其用于不同聚合物基质中复合SPF的合成。聚合物基质中的SPF对于将SERS基底常规用于各种类型的分析物（包括固体样品或对污染敏感的样品）是必要的。研究了聚苯乙烯、聚乙烯醇（PVA）和聚乙烯，以获得用于SPF的聚合物基质，并正在探索将SPF掺入聚合物基质的各种方法。发现聚苯乙烯中获得的薄膜具有最佳的信号增强和重现性。所获得的SERS基底的最低可检测浓度等于10⁻⁶ M。我们制备了一种分析增强因子为2.7×10⁶的SERS基底，可使分析物溶液的检测灵敏度提高五个数量级。
