基于表面增强拉曼散射的侧向流分析平台对丛林斑疹伤寒的定量血清诊断。

Quantitative Serodiagnosis of Scrub Typhus Using Surface-Enhanced Raman Scattering-Based Lateral Flow Assay Platforms.

机构信息

Department of Chemistry , Chung-Ang University , Seoul 06974 , South Korea.

SG Medical, Inc. , Seoul 05548 , South Korea.

出版信息

Anal Chem. 2019 Oct 1;91(19):12275-12282. doi: 10.1021/acs.analchem.9b02363. Epub 2019 Aug 12.

DOI:10.1021/acs.analchem.9b02363

PMID:31356055

Abstract

A surface-enhanced Raman scattering-based lateral flow assay (SERS-LFA) technique has been developed for the rapid and accurate diagnosis of scrub typhus. Lateral flow kits for the detection of IgG (scrub typhus biomarker) were fabricated, and the calibration curve for various standard clinical sera concentrations were obtained by Raman measurements. The clinical sera titer values were determined by fitting the Raman data to the calibration curve. To assess the clinical feasibility of the proposed method, SERS-LFA assays were performed on 40 clinical samples. The results showed good agreement with those of the standard indirect immunofluorescence assay (IFA) method. SERS-LFA has many advantages over IFA including the less sample volume, simpler assay steps, shorter assay time, more systematic quantitative analysis, and longer assay lifetime. As SERS strips can be easily integrated with a miniaturized Raman spectrophotometer, field serodiagnosis is also more feasible.

摘要

一种基于表面增强拉曼散射的侧向流动检测技术（SERS-LFA）已被开发出来，用于快速、准确地诊断恙虫病。侧向流动试剂盒用于检测 IgG（恙虫病生物标志物），通过拉曼测量获得各种标准临床血清浓度的校准曲线。通过将拉曼数据拟合到校准曲线上，可以确定临床血清滴度值。为了评估该方法的临床可行性，对 40 个临床样本进行了 SERS-LFA 检测。结果与标准间接免疫荧光法（IFA）的检测结果吻合良好。与 IFA 相比，SERS-LFA 具有许多优势，包括样品量更少、检测步骤更简单、检测时间更短、更系统的定量分析以及更长的检测寿命。由于 SERS 条很容易与微型拉曼分光光度计集成，因此现场血清诊断也更加可行。