National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Ngaoundere P.O. Box 454, Cameroon.
Int J Mol Sci. 2022 Jul 26;23(15):8208. doi: 10.3390/ijms23158208.
Dwarfing is important for the production of wheat ( L.). In model plants, receptor-like kinases have been implicated in signal transduction, immunity, and development. However, functional roles of lectin receptor-like kinases in wheat are poorly understood. In this study, we identified an L-type lectin receptor-like kinase gene in wheat, designated as , and revealed its role in plant height. Real time quantitative PCR analyses indicated that transcript level was lower in a dwarf wheat line harboring the gene compared to its transcript level detected in a taller wheat line CI12633. Importantly, the virus-induced gene silencing results showed that silencing of in the wheat line CI12633 led to dwarf plants. The results of the disease resistance test performed after the gene silencing experiment suggest no significant role of in the resistance reaction of wheat line CI12633 to sharp eyespot. Gene expression analysis revealed that the transcript abundance of was more up-regulated after the exogenous application of gibberellic acid and auxin, two development-related phytohormones, compared to the gene transcript levels detected in the control plants (mock treatment). These findings support the potential implication of in the pathway controlling plant height rather than the disease resistance role, and suggest that may be a positive regulator of plant height through the gibberellic acid and auxin-signaling pathways.
矮化对于小麦（L.）的生产很重要。在模式植物中，受体样激酶已被牵涉到信号转导、免疫和发育中。然而，凝集素受体样激酶在小麦中的功能作用还知之甚少。在本研究中，我们在小麦中鉴定出一个 L 型凝集素受体样激酶基因，命名为 ，并揭示了它在株高中的作用。实时定量 PCR 分析表明，在含有 基因的矮秆小麦品系中， 的转录水平低于在更高秆小麦品系 CI12633 中检测到的转录水平。重要的是，病毒诱导的基因沉默结果表明，在小麦品系 CI12633 中沉默 导致矮化植株。基因沉默实验后的抗病性测试结果表明， 在小麦品系 CI12633 对尖锐眼斑的抗性反应中没有重要作用。基因表达分析显示，与对照植物（模拟处理）相比，外源施用赤霉素和生长素这两种发育相关的植物激素后， 的转录丰度上调更为明显。这些发现支持了 在控制株高的途径中可能具有潜在的作用，而不是抗病作用，并表明 可能通过赤霉素和生长素信号通路成为株高的正调节剂。
