Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Int J Mol Sci. 2023 Jun 21;24(13):10421. doi: 10.3390/ijms241310421.
Plant height is one of the key agronomic traits for improving the yield of sweet potato. Phytohormones, especially gibberellins (GAs), are crucial to regulate plant height. The enzyme 9--epoxycarotenoid dioxygenase (NCED) is the key enzyme for abscisic acid (ABA) biosynthesis signalling in higher plants. However, its role in regulating plant height has not been reported to date. Here, we cloned a new NCED gene, , from the sweet potato cultivar Jishu26. This gene encoded the 587-amino acid polypeptide containing an NCED superfamily domain. The expression level of was highest in the stem and the old tissues in the in vitro-grown and field-grown Jishu26, respectively. The expression of was induced by ABA and GA3. Overexpression of promoted the accumulation of ABA and inhibited the content of active GA3 and plant height and affected the expression levels of genes involved in the GA metabolic pathway. Exogenous application of GA3 could rescue the dwarf phenotype. In conclusion, we suggest that regulates plant height and development by controlling the ABA and GA signalling pathways in transgenic sweet potato.
株高是提高甘薯产量的主要农艺性状之一。植物激素，尤其是赤霉素（GA），对调节株高至关重要。9--环氧类胡萝卜素双加氧酶（NCED）是高等植物脱落酸（ABA）生物合成信号转导的关键酶。然而，迄今为止，其在调节株高方面的作用尚未有报道。在这里，我们从甘薯品种 Jishu26 中克隆了一个新的 NCED 基因 。该基因编码含有 NCED 超家族结构域的 587 个氨基酸多肽。在体外培养和田间生长的 Jishu26 中， 的表达水平在茎和老组织中最高。 的表达受 ABA 和 GA3 的诱导。过表达 促进 ABA 的积累，抑制活性 GA3 的含量和株高，并影响参与 GA 代谢途径的基因的表达水平。外源 GA3 的应用可以挽救矮化表型。综上所述，我们认为 通过控制 ABA 和 GA 信号通路来调节转基因甘薯的株高和发育。
