Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033, Japan.
Technology Research Laboratory, Shimadzu Corporation, Kyoto 619-0237, Japan.
Molecules. 2022 Jul 23;27(15):4715. doi: 10.3390/molecules27154715.
In our previous study, we developed an automatic sample injection system for pillar array columns for quantitative analysis. An autosampler was used to maintain a constant sample injection volume. However, the sample was diluted during injection using the autosampler, thus deteriorating the analytical reproducibility. In this study, we have substituted the autosampler with a syringe pump to overcome the abovementioned problem and improve the system. Sample dilution was avoided by filling the entire capillary with the sample at a constant rate. This improved system also increased the analytical reproducibility. In the previous system, the relative standard deviation (RSD) exceeded 17% of the peak height for coumarin dyes. In contrast, the improved system decreased the RSD to the range 1.2-1.8%. The analytical reproducibility was evaluated by using five types of amino acids. The RSD of each peak height was within 3.0%, confirming good reproducibility. These results indicate that the sample injection method developed in this study can be applied to biological sample analyses as a simple quantitative analysis method for pillar array columns.
在我们之前的研究中，我们开发了一种用于柱列阵列的自动进样系统，用于定量分析。进样器用于保持恒定的进样体积。然而，进样过程中，样品会被进样器稀释，从而降低了分析重现性。在这项研究中，我们用注射器泵取代了进样器，以克服上述问题并改进系统。通过以恒定的速度将整个毛细管充满样品，避免了样品稀释。该改进后的系统还提高了分析重现性。在前一个系统中，香豆素染料的峰高相对标准偏差（RSD）超过了 17%。相比之下，改进后的系统将 RSD 降低到 1.2-1.8%。使用五种类型的氨基酸评估了分析重现性。每个峰高的 RSD 均在 3.0%以内，表明重现性良好。这些结果表明，本研究中开发的进样方法可作为一种简单的定量分析方法，应用于柱列阵列的生物样品分析。
