Kakatiya Institution of Pharmaceutical Sciences, Hanamkonda, Telangana, India.
Pathfinder Institute of Pharmacy Education and Research, Kakatiya University, Warangal, Telangana, India.
Adv Exp Med Biol. 2021;1339:59-63. doi: 10.1007/978-3-030-78787-5_9.
Flurbiprofen (FLU), a chiral 2-arylpropionic acid (2-APA) nonsteroidal anti-inflammatory drug (NSAID), is used in its racemic form. Enantiomers are optically active compounds with one or more chiral centers and have identical physicochemical properties except the rotation of plane polarized light. The enantiomers of 2-APAs, however, may exert different pharmacodynamic and pharmacokinetic effects. Several methods have been reported for the detection of racemic FLU in plasma, i.e., thin-layer chromatography, gas chromatography, and high-performance liquid chromatography. The method involves extraction of drug and ibuprofen (internal standard, IS) with a mixture of isooctane and 2-propanol. The described stereoselective, specific, HPLC method provides a simple, sensitive, and reliable approach for the determination of the enantiomers of FLU in plasma after clinical doses, which is applicable for routine analysis.
氟比洛芬（FLU）是一种手性 2-芳基丙酸（2-APA）类非甾体抗炎药（NSAID），以外消旋体形式使用。对映异构体是具有一个或多个手性中心的光学活性化合物，除了平面偏振光的旋转外，具有相同的物理化学性质。然而，2-APAs 的对映异构体可能发挥不同的药效学和药代动力学作用。已经报道了几种用于检测血浆中外消旋 FLU 的方法，例如薄层色谱法、气相色谱法和高效液相色谱法。该方法涉及用异辛烷和 2-丙醇的混合物提取药物和布洛芬（内标，IS）。所描述的立体选择性、特异性、HPLC 方法为临床剂量后血浆中 FLU 对映异构体的测定提供了一种简单、灵敏和可靠的方法，适用于常规分析。
