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基于叶片损伤的表型分析技术及其针对玉米中草地贪夜蛾(J.E. 史密斯)的验证

Leaf Damage Based Phenotyping Technique and Its Validation Against Fall Armyworm, (J. E. Smith), in Maize.

作者信息

Soujanya P Lakshmi, Sekhar J C, Yathish K R, Karjagi Chikkappa G, Rao K Sankara, Suby S B, Jat S L, Kumar Bhupender, Kumar Krishan, Vadessery Jyothilakshmi, Subaharan K, Patil Jagadish, Kalia Vinay K, Dhandapani A, Rakshit Sujay

机构信息

Winter Nursery Centre, ICAR-Indian Institute of Maize Research, Hyderabad, India.

Unit Office, ICAR-Indian Institute of Maize Research, New Delhi, India.

出版信息

Front Plant Sci. 2022 Jul 11;13:906207. doi: 10.3389/fpls.2022.906207. eCollection 2022.

DOI:10.3389/fpls.2022.906207
PMID:35898223
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9310382/
Abstract

Globally, maize is an important cereal food crop with the highest production and productivity. Among the biotic constraints that limit the productivity of maize, the recent invasion of fall armyworm (FAW) in India is a concern. The first line of strategy available for FAW management is to evaluate and exploit resistant genotypes for inclusion in an IPM schedule. Screening for resistant maize genotypes against FAW is in its infancy in India, considering its recent occurrence in the country. The present work attempts to optimize screening techniques suited to Indian conditions, which involve the description of leaf damage rating (LDR) by comparing injury levels among maize genotypes and to validate the result obtained from the optimized screening technique by identification of lines potentially resistant to FAW under artificial infestation. Exposure to 20 neonate FAW larvae at the V phenological stage coupled with the adoption of LDR on a 1-9 scale aided in preliminary characterize maize genotypes as potentially resistant, moderately resistant, and susceptible. The LDR varies with genotype, neonate counts, and days after infestation. The genotypes, ., DMRE 63, DML-163-1, CML 71, CML 141, CML 337, CML 346, and wild ancestor ssp. recorded lower LDR ratings against FAW and can be exploited for resistance breeding in maize.

摘要

在全球范围内,玉米是产量和生产率最高的重要谷类粮食作物。在限制玉米生产率的生物胁迫因素中,印度最近入侵的草地贪夜蛾令人担忧。草地贪夜蛾管理的首要策略是评估和利用抗性基因型,将其纳入综合虫害管理计划。鉴于草地贪夜蛾最近才在印度出现,针对该害虫筛选抗性玉米基因型的工作在印度尚处于起步阶段。目前的工作旨在优化适合印度条件的筛选技术,包括通过比较玉米基因型之间的受害程度来描述叶片损伤评级(LDR),并通过在人工接种条件下鉴定对草地贪夜蛾具有潜在抗性的品系,来验证从优化筛选技术中获得的结果。在V物候期让20头草地贪夜蛾初孵幼虫接触玉米,并采用1-9级的叶片损伤评级,有助于初步将玉米基因型表征为潜在抗性、中度抗性和易感型。叶片损伤评级随基因型、幼虫数量和接种后天数而变化。DMRE 63、DML-163-1、CML 71、CML 141、CML 337、CML 346等基因型以及野生祖先亚种对草地贪夜蛾的叶片损伤评级较低,可用于玉米的抗性育种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/971f/9310382/bf3925a44178/fpls-13-906207-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/971f/9310382/7ead553b4225/fpls-13-906207-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/971f/9310382/6259582ba3fc/fpls-13-906207-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/971f/9310382/bf3925a44178/fpls-13-906207-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/971f/9310382/7ead553b4225/fpls-13-906207-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/971f/9310382/6259582ba3fc/fpls-13-906207-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/971f/9310382/bf3925a44178/fpls-13-906207-g0003.jpg

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