生物信息学分析鉴定 2 型糖尿病胰岛功能障碍中的潜在铁死亡关键基因。
Bioinformatics Analysis Identifies Potential Ferroptosis Key Gene in Type 2 Diabetic Islet Dysfunction.
机构信息
Department of Endocrinology and Metabolism, First Affiliated Hospital of Jinan University, Guangzhou, China.
Clinical Experimental Center, First Affiliated Hospital of Jinan University, Guangzhou, China.
出版信息
Front Endocrinol (Lausanne). 2022 Jul 11;13:904312. doi: 10.3389/fendo.2022.904312. eCollection 2022.
BACKGROUND
Islet β cells dysfunction (IBCD) is a cortical component in pathogenesis of type 2 diabetic mellitus (T2DM). However, the relationship of ferroptosis and IBCD remains unknown. This study was aimed to screen potential ferroptosis key genes to reveal latent physiological and pathological process of IBCD in T2DM.
METHODS
Firstly, T2DM key genes were screened by combining with differentially expressed genes (DEGs) analysis and WGCNA. Then, ferroptosis-related genes (FRGs) in IBCD of T2DM were identified by taking the intersection between T2DM key genes and FRGs. Finally, T2DM-FRGs were validated in another T2DM dataset as well as islet single-cell RNA sequencing dataset and the miRNA regulated T2DM-FRG was predicted by using four miRNA databases.
RESULTS
89 T2DM key genes were identified between DEGs and WGCNA. Then, 3 T2DM-FRGs were screened by taking the intersection of T2DM key genes and FRGs, namely , and . At last, were validated as the T2DM-FRG in another T2DM islet issues dataset and islet single-cell RNA sequencing dataset.
CONCLUSION
may be the potential ferroptosis key gene of IBCD in T2DM.
背景
胰岛 β 细胞功能障碍（IBCD）是 2 型糖尿病（T2DM）发病机制中的皮质组成部分。然而，铁死亡与 IBCD 的关系尚不清楚。本研究旨在筛选潜在的铁死亡关键基因，以揭示 T2DM 中 IBCD 的潜在生理和病理过程。
方法
首先，通过结合差异表达基因（DEGs）分析和 WGCNA 筛选 T2DM 关键基因。然后，通过取 T2DM 关键基因与铁死亡相关基因（FRGs）的交集，确定 T2DM 中 IBCD 的铁死亡相关基因（FRGs）。最后，在另一个 T2DM 数据集以及胰岛单细胞 RNA 测序数据集中验证 T2DM-FRGs，并使用四个 miRNA 数据库预测受 miRNA 调控的 T2DM-FRG。
结果
在 DEGs 和 WGCNA 之间确定了 89 个 T2DM 关键基因。然后，通过取 T2DM 关键基因与 FRGs 的交集，筛选出 3 个 T2DM-FRGs，分别为、和。最后，在另一个 T2DM 胰岛组织数据集和胰岛单细胞 RNA 测序数据集中验证了为 T2DM-FRG。
结论
可能是 T2DM 中 IBCD 的潜在铁死亡关键基因。
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