Chimeric RNA TNNI2-ACTA1-V1 Regulates Cell Proliferation by Regulating the Expression of NCOA3.

Chimeric RNA is a crucial target for tumor diagnosis and drug therapy, also having its unique biological role in normal tissues. (), a chimeric RNA discovered by our laboratory in porcine muscle tissue, can inhibit the proliferation of Porcine Skeletal Muscle Satellite Cells (PSCs). The regulatory mechanism of in PSCs remains unclear, but we speculate that and may be the target genes of . In this study, we explored the effects of and on cell viability and cell proliferation by CCK-8 assay, EdU staining and flow cytometry. Furthermore, the regulatory pathway of proliferation in PSCs mediated by through or was elucidated by co-transfection and co-immunoprecipitation (Co-IP). The results revealed that overexpression of significantly increased cell viability and the expression level of , and also promotes cell proliferation by changing cells from the G1 phase to the S phase. In addition, inhibiting the expression of substantially reduced cell viability and inhibited cell proliferation. Overexpression of and had no significant effect on cell viability and proliferation. Co-transfection experiments showed that could rescue the proliferation inhibition of PSCs caused by . Co-IP assay indicated that directly interacts with . Our study explores the hypothesis that directly regulates , indirectly regulating , thereby regulating PSCs proliferation. We provide new putative mechanisms of porcine skeletal muscle growth and lay the foundation for the study of chimeric RNA in normal tissues.