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[污水中新型冠状病毒2019的富集及核酸检测方法]

[Methods for enrichment and nucleic acid detection of SARS-CoV-2 in sewage].

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2022 Jul 6;56(7):891-896. doi: 10.3760/cma.j.cn112150-20220420-00396.

Abstract

Since the outbreak of COVID-19, SARS-CoV-2 surveillance in sewage based on sewage epidemiology has been established in many countries around the world, while there is a lack of a standard detection method for SARS-CoV-2 in sewage in China. This standard provides three methods for the enrichment of SARS-CoV-2 in sewage, namely polyethylene glycol precipitation, aluminum salt coagulation precipitation and centrifugal ultrafiltration, which are suitable for the enrichment and nucleic acid detection of SARS-CoV-2 in domestic sewage and sewage from medical institutions. This standard is of great significance for standardizing the SARS-CoV-2 detection in sewage and ensuring the scientific and effective detection of SARS-CoV-2 in sewage.

摘要

自新冠疫情爆发以来,世界上许多国家已基于污水流行病学开展了污水中新型冠状病毒(SARS-CoV-2)监测工作,而我国缺乏污水中SARS-CoV-2的标准检测方法。本标准提供了三种污水中SARS-CoV-2的富集方法,即聚乙二醇沉淀法、铝盐混凝沉淀法和离心超滤法,适用于生活污水和医疗机构污水中SARS-CoV-2的富集及核酸检测。本标准对于规范污水中SARS-CoV-2检测、确保污水中SARS-CoV-2检测科学有效具有重要意义。

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