Department of Clinical pharmacy, Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou, 563000, China.
Department of Laboratory Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, 563003, China.
Food Funct. 2022 Aug 15;13(16):8643-8651. doi: 10.1039/d2fo01719a.
Alcohol is one of the most commonly used addictive substances. Addiction memory is a necessary part of the mechanism underlying drug addiction and its extract can mitigate organ damage caused by alcohol. In this study, polysaccharide (LJP) was extracted, and its effect on alcohol addiction memory was investigated. LJP inhibited the cue-induced reinstatement of conditioned place preference and elevated Tuj1 and DCX levels in the hippocampus to suppress neuronal damage. LJP also reduced the hippocampal glutamate (Glu) levels, alcohol-induced abnormal enhancement of the addiction memory, and decreased VPS34 phosphorylation and hyper activation of autophagy pathways in the hippocampus of alcohol-dependent mice. Our results suggest that LJP is a potential functional food to treat or ameliorate alcohol-induced addiction and that VPS34 is a potential target for modulating alcohol cravings.
酒精是最常用的成瘾物质之一。成瘾记忆是药物成瘾机制的必要组成部分，其提取物可以减轻酒精引起的器官损伤。在这项研究中，提取了多糖（LJP），并研究了其对酒精成瘾记忆的影响。LJP 抑制了线索诱导的条件性位置偏好的恢复，并在海马体中升高了 Tuj1 和 DCX 的水平，从而抑制神经元损伤。LJP 还降低了海马体谷氨酸（Glu）水平，减轻了酒精引起的成瘾记忆异常增强，并减少了酒精依赖小鼠海马体中 VPS34 磷酸化和自噬途径的过度激活。我们的研究结果表明，LJP 是一种治疗或改善酒精引起的成瘾的潜在功能性食品，VPS34 是调节酒精渴求的潜在靶点。
