Liu Ping, Bai Xinyu, Zhang Tao, Zhou Limei, Li Jing, Zhang Lan
Department of Pharmacy, Xuanwu Hospital of Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing Engineering Research Center for Nervous System Drugs, Beijing Institute for Brain Disorders, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China.
Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563000, China.
Ann Transl Med. 2019 Dec;7(24):811. doi: 10.21037/atm.2019.12.64.
Depression is associated with the abnormal activation of the human inflammatory response system, which is a life-threatening disorder affecting millions of people of all ages around the world. The purposes of the present study were to examine the effect of polysaccharide (LJP), a polysaccharide extracted from Thunb., on depressed mice treated with the unpredictable emotional stress stimulation.
The components of LJP after extraction were detected by HPLC. Depression model is established through chronic unpredictable stimulation, and the depression behavior was assessed by mouse behavioral assessment, including open field, elevated plus maze, tail suspension, forced swim. Pathological changes in hippocampus of mice were observed by HE and toluidine blue staining. Protein expression of NLRP3 inflammasome pathway was detected by WB.
It was showed that LJP mainly consisted of 8.7% GalA, 8.2% Rha, 16.2% Gal, 19.5% Ara, 26.9% Glc and 20.5% Man, with the average molecular weight approximately ranging from 1 to 1,000 kDa, which could significantly reduce the time in the open arms and the immobility time of the depressed mice in behavioral tests, and the expressions of NLRP3, IL-1β, and caspase-1 in the hippocampus of depressed mice were significantly upregulated.
LJP exhibited a strong protective effect on mice with depression by inhibiting NLRP3 inflammasome. The results will help to understand the potential use of Lonicera japonica Thun polysaccharide in pharmaceutical and food applications better.
抑郁症与人类炎症反应系统的异常激活有关，这是一种危及生命的疾病，影响着全球数百万各年龄段的人。本研究的目的是研究从忍冬中提取的多糖（LJP）对接受不可预测情绪应激刺激的抑郁小鼠的影响。
采用高效液相色谱法检测LJP提取后的成分。通过慢性不可预测刺激建立抑郁模型，通过小鼠行为学评估（包括旷场试验、高架十字迷宫试验、悬尾试验、强迫游泳试验）评估抑郁行为。通过苏木精-伊红（HE）染色和甲苯胺蓝染色观察小鼠海马体的病理变化。通过蛋白质免疫印迹法检测NLRP3炎性小体通路的蛋白表达。
结果显示，LJP主要由8.7%的半乳糖醛酸（GalA）、8.2%的鼠李糖（Rha）、16.2%的半乳糖（Gal）、19.5%的阿拉伯糖（Ara）、26.9%的葡萄糖（Glc）和20.5%的甘露糖（Man）组成，平均分子量约为1至1000 kDa，其可显著减少行为测试中抑郁小鼠在开放臂的停留时间和不动时间，且抑郁小鼠海马体中NLRP3、白细胞介素-1β（IL-1β）和半胱天冬酶-1（caspase-1）的表达显著上调。
LJP通过抑制NLRP3炎性小体对抑郁小鼠表现出强大的保护作用。这些结果将有助于更好地理解忍冬多糖在制药和食品应用中的潜在用途。
