Hsa_circ_0008092 通过 miR-502-5p/CCND1 轴促进肝癌细胞增殖和转移。

Hsa_circ_0008092 Contributes to Cell Proliferation and Metastasis in Hepatocellular Carcinoma via the miR-502-5p/CCND1 Axis.

机构信息

Clinic of Medical Department, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang, China.

Clinic of Medical Department, the First Affiliated Hospital of Xinjiang Medical University, 830000, Xinjiang, China.

出版信息

Protein Pept Lett. 2022;29(7):595-604. doi: 10.2174/0929866529666220721090209.

DOI:10.2174/0929866529666220721090209

PMID:35899951

Abstract

BACKGROUND

The present study was targeted at investigating the effects of hsa_circRNA_0008092 (circ_0008092) on hepatocellular carcinoma (HCC) cell proliferation, migration, invasion and apoptosis, and its related mechanism.

METHODS

The gene expression profiles of GSE166678 were downloaded from the Gene Expression Omnibus database, and differentially expressed circRNAs in human HCC were screened out. Besides, circ_0008092, microRNA-502-5p (miR-502-5p) and cyclin D1 (CCND1) expressions in HCC tissues and cell lines were detected by quantitative real-time polymerase chain reaction (qRTPCR). Cell countering kit-8 (CCK-8), Transwell and flow cytometry assays were used to detect the proliferation, migration, invasion and apoptosis of HCC cells. Bioinformatics was utilized to predict the targeted relationships between miR-502-5p and circ_0008092, as well as miR-502-5p and CCND1 mRNA 3'-untranslated region (3'UTR). Western blot assay was applied to detect CCND1 protein expression in HCC cells.

RESULTS

Circ_0008092 was highly expressed in HCC tissues and cells, which was associated with a shorter survival time in patients with HCC. Circ_0008092 overexpression promoted proliferation, migration and invasion, and inhibited apoptosis of HCC cells; circ_0008092 knockdown worked oppositely. Circ_0008092 directly targeted miR-502-5p and negatively modulated miR-502-5p expression. CCND1 Conclusion: Our data suggest that circ_0008092 promotes HCC progression by regulating the miR- 502-5p/CCND1 axis.

摘要

背景

本研究旨在探讨 hsa_circRNA_0008092（circ_0008092）对肝细胞癌（HCC）细胞增殖、迁移、侵袭和凋亡的影响及其相关机制。

方法

从基因表达综合数据库中下载 GSE166678 基因表达谱，筛选出人 HCC 中差异表达的 circRNA。此外，采用实时定量聚合酶链反应（qRT-PCR）检测 HCC 组织和细胞系中 circ_0008092、微小 RNA-502-5p（miR-502-5p）和细胞周期蛋白 D1（CCND1）的表达。采用细胞计数试剂盒-8（CCK-8）、Transwell 和流式细胞术检测 HCC 细胞的增殖、迁移、侵袭和凋亡。利用生物信息学预测 miR-502-5p 与 circ_0008092 以及 miR-502-5p 与 CCND1 mRNA 3'-非翻译区（3'-UTR）的靶向关系。Western blot 检测 HCC 细胞中 CCND1 蛋白的表达。

结果

circ_0008092 在 HCC 组织和细胞中高表达，与 HCC 患者的生存时间较短相关。circ_0008092 过表达促进 HCC 细胞的增殖、迁移和侵袭，抑制凋亡；circ_0008092 敲低则作用相反。circ_0008092 可直接靶向 miR-502-5p，负调控 miR-502-5p 的表达。CCND1