WISP1通过促进细胞周期蛋白D1泛素化并下调其表达来抑制肝癌细胞增殖。
WISP1 Inhibits Hepatocellular Carcinoma Cell Proliferation by Promoting CyclinD1 Ubiquitination and Downregulating its Expression.
作者信息
Yan Jinlong, Hu Jun Wen, Cheng Na, Li Nuoya, Xin Anqi, Wu Zhipeng, Wu Zhengyi, Lei Jun, Zhang Shouhua, Yao Jinping
机构信息
Department of General Surgery, The Second AffiliatedHospital, Jiangxi Medical College, Nanchang University, Jiangxi, China.
Department of Hospital Infection Control, The First Affiliated Hospital of Nanchang University, Jiangxi, China.
出版信息
Turk J Gastroenterol. 2024 Dec 16;36(4):247-254. doi: 10.5152/tjg.2024.23524.
BACKGROUND/AIMS: Hepatocellular carcinoma (HCC), a leading cause of cancer-related deaths, is often linked to dysregulated cell cycle proteins. This study focuses on the role of WISP1 in modulating Cyclin D1, a key cell cycle regulator, in HCC.
MATERIALS AND METHODS
The study used HCCLM3 and Hep3B cells to assess the expression of Cyclin D1 and cell proliferation following the treatment of WISP1. This was achieved through Western blot, qRT-PCR, and EdU assays. Additionally, animal studies were conducted to evaluate the effects of WISP1 treatment on Cyclin D1 expression and cell proliferation.
RESULTS
Overexpression of WISP1 in HCC cells led to a marked decrease in Cyclin D1 protein levels and reduced cell proliferation. WISP1 influences Cyclin D1 through post-translational modifications, particularly ubiquitination and proteasomal degradation.
CONCLUSION
The findings revealed that WISP1’s modulation of Cyclin D1 plays a critical role in inhibiting HCC cell growth, highlighting a potential therapeutic target for HCC treatment.
背景/目的：肝细胞癌（HCC）是癌症相关死亡的主要原因之一，常与细胞周期蛋白失调有关。本研究聚焦于WISP1在肝癌中调节细胞周期关键调节因子细胞周期蛋白D1（Cyclin D1）的作用。
材料与方法
本研究使用HCCLM3和Hep3B细胞来评估WISP1处理后细胞周期蛋白D1的表达及细胞增殖情况。这通过蛋白质免疫印迹法、定量逆转录聚合酶链反应和5-乙炔基-2'-脱氧尿苷检测来实现。此外，还进行了动物研究以评估WISP1处理对细胞周期蛋白D1表达和细胞增殖的影响。
结果
肝癌细胞中WISP1的过表达导致细胞周期蛋白D1蛋白水平显著降低，并减少细胞增殖。WISP1通过翻译后修饰，特别是泛素化和蛋白酶体降解来影响细胞周期蛋白D1。
结论
研究结果表明，WISP1对细胞周期蛋白D1的调节在抑制肝癌细胞生长中起关键作用，凸显了其作为肝癌治疗潜在靶点的可能性。
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