基于 AuCu 双金属纳米簇和 MoS 的脱氧雪腐镰刀菌烯醇荧光适体传感器

Deoxynivalenol fluorescence aptasensor based on AuCu bimetallic nanoclusters and MoS.

机构信息

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.

School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.

出版信息

Mikrochim Acta. 2022 Jul 28;189(8):296. doi: 10.1007/s00604-022-05385-4.

DOI:10.1007/s00604-022-05385-4

PMID:35900604

Abstract

Aptamers against deoxynivalenol (DON) were selected through capture-systematic evolution of ligands by exponential enrichment. Through isothermal titration calorimetry and fluorimetric assay, aptamer candidate DN-2 demonstrated good affinity to DON with K value of 40.36 ± 6.32 nM. Accordingly, a Forster resonance energy transfer aptasensor was fabricated by using the aptamer DN-2 combined with AuCu bimetallic nanoclusters as energy donor and MoS nanosheets as energy acceptor. Under the optimal conditions, the fluorescence response was utilized for DON quantitative determination ranging from 5 to 100 ng/mL with a detection limit of 1.87 ng/mL. The practical application of this method was verified in maize flour samples and demonstrated a satisfied recovery of 94.6 ~ 103.1%. The obtained aptamers and their application in DON determination provide a new tool for DON monitoring in various foodstuff.

摘要

通过指数富集配体系统进化（SELEX）技术筛选得到脱氧雪腐镰刀菌烯醇（DON）适配体。通过等温滴定量热法和荧光检测法，适配体候选物 DN-2 对 DON 表现出良好的亲和力，其 K 值为 40.36 ± 6.32 nM。因此，利用该适配体 DN-2 与 AuCu 双金属纳米簇作为能量供体和 MoS 纳米片作为能量受体，构建了一种基于Förster 共振能量转移（FRET）的适配体传感器。在最佳条件下，该荧光响应可用于 DON 的定量检测，检测范围为 5 至 100 ng/mL，检测限为 1.87 ng/mL。该方法在玉米粉样品中的实际应用验证了其具有令人满意的回收率，为 94.6%至 103.1%。该适配体的获得及其在 DON 测定中的应用为各种食品中 DON 的监测提供了一种新工具。