Anderson E, Little B, Lee G S
Tissue Cell. 1987;19(2):217-34. doi: 10.1016/0040-8166(87)90007-3.
Ovarian granulosa cells collected from small antral follicles from immature rats were cultured in McCoy's 5A medium, for 1-6 days in the presence of delta 4-androstenedione, testosterone, dihydrotestosterone, and dehydroepiandrosterone (10(-5) M and 10(-7) M). Granulosa cells examined by electron microscopy demonstrated many lipid droplets, mitochondria with tubular cristae and profiles of smooth endoplasmic reticulum, all suggestive of active metabolism in the cell. Cells cultured in androstenedione, testosterone, dihydrotestosterone and dehydroepiandrosterone produced estrogen and progesterone as measured by radioimmunoassay. By day 4, cells cultured in androgen had almost completely degenerated. The control cells acquired none of the aforementioned characteristics and survived up to beyond 6 days, at which time the experiments were terminated. This study supports the hypothesis that high concentrations of androgens in cultured granulosa cells contribute to their degeneration through altered structure, which is associated with functional change.
从未成熟大鼠小窦状卵泡中收集的卵巢颗粒细胞，在含有Δ4-雄烯二酮、睾酮、双氢睾酮和脱氢表雄酮（10⁻⁵ M和10⁻⁷ M）的 McCoy's 5A 培养基中培养1至6天。通过电子显微镜检查的颗粒细胞显示出许多脂滴、具有管状嵴的线粒体和平滑内质网的轮廓，所有这些都表明细胞内代谢活跃。通过放射免疫测定法测量，在雄烯二酮、睾酮、双氢睾酮和脱氢表雄酮中培养的细胞产生了雌激素和孕酮。到第4天，在雄激素中培养的细胞几乎完全退化。对照细胞没有获得上述任何特征，存活超过6天，此时实验终止。本研究支持以下假设：培养的颗粒细胞中高浓度雄激素通过改变结构导致其退化，这与功能变化有关。
