Polymorphism of ACP1 by isoelectric focusing: population study in Japanese and phenotyping in bloodstains.

The polymorphism of ACP1 was investigated in 749 unrelated Japanese individuals by isoelectric focusing. The allele frequencies were ACP1* A = 0.2083 and ACP1* B = 0.7917, which were almost similar to those reported in other Japanese subpopulations. The isoelectric focusing technique was successfully applied to ACP1 typing in bloodstains; each phenotype was demonstrated at 37 degrees C for up to 4 weeks, at room temperature for up to 10 weeks and at 4 degrees C for over 16 weeks after stain formation. The isoelectric focusing method proved more useful than conventional electrophoretic methods in medicolegal practice.