Comparing fixed-array and functionally-defined channel of interest approaches to infant functional near-infrared spectroscopy data.

Functional near-infrared spectroscopy (fNIRS) is increasingly used to study brain function in infants, but the development and standardization of analysis techniques for use with infant fNIRS data have not paced other technical advances. Here we quantify and compare the effects of different methods of analysis of infant fNIRS data on two independent fNIRS datasets involving 6-9-month-old infants and a third simulated infant fNIRS dataset. With each, we contrast results from a traditional, fixed-array analysis with several functional channel of interest (fCOI) analysis approaches. In addition, we tested the effects of varying the number and anatomical location of potential data channels to be included in the fCOI definition. Over three studies we find that fCOI approaches are more sensitive than fixed-array analyses, especially when channels of interests were defined within-subjects. Applying anatomical restriction and/or including multiple channels in the fCOI definition does not decrease and in some cases increases sensitivity of fCOI methods. Based on these results, we recommend that researchers consider employing fCOI approaches to the analysis of infant fNIRS data and provide some guidelines for choosing between particular fCOI approaches and settings for the study of infant brain function and development.