Examining sex disparities in risk/reward trade-offs in Smith's zokors, Eospalax smithii.

Risk taking is imperative for the survival and fitness of animals since they are constantly facing innumerable threats from various sources. Indeed, the ability of the individual to balance the costs and benefits of various options and adopt a wise decision is critical for the animal well-being. We modified several traditionally used anxiety tests [The modified light-dark box (mLDB), the modified open field test (mOFT) and the modified defensive withdrawal apparatus (mDWA)] by adding a palatable food reward within the anxiogenic zone which granted us to assess the sex differences in risk-taking behavior in Smith's zokors (Eospalax smithii), a typical subterranean rodent species endemic to the Qinghai-Tibetan Plateau. Concomitant with our working hypothesis, female zokors showed strong aversion and avoidance behavioral responses when tested in the mOFT and mDWA while there were no apparent sexually dimorphic behavioral changes when they were tested in the mLDB (Except for the percentage of food consumed and the latency till start feeding). Furthermore, comparison between the three behavioral paradigms revealed that both sexes showed different behavioral responses toward the different behavioral tests. Sex differences in repeatable behaviors were more profound in females than males. This might reflect different degrees of risk perception and emotionality that may differ considerably between the different models of anxiety. Our results highlighted the functional significance of a trade-off between risk and incentives in natural environment that both male and female zokors differ in the processing of risk assessment in the presence of a food reward.