Laboratory of Animal Reproduction and Physiology, Division of Animal and Dairy Science, College of Agriculture and Life Science, Chungnam National University, Daejeon, 34134, Korea.
MK Biotech Inc., Daejeon, 34134, Korea.
Sci Rep. 2022 Jul 28;12(1):12905. doi: 10.1038/s41598-022-17200-4.
Canine hip dysplasia (HD) is a multifactorial disease caused by interactions between genetic and environmental factors. HD, which mainly occurs in medium- to large-sized dogs, is a disease that causes severe pain and requires surgical intervention. However, the procedure is not straight-forward, and the only way to ameliorate the situation is to exclude individual dogs with HD from breeding programs. Recently, prime editing (PE), a novel genome editing tool based on the CRISPR-Cas9 system, has been developed and validated in plants and mice. In this study, we successfully corrected a mutation related to HD in Labrador retriever dogs for the first time. We collected cells from a dog diagnosed with HD, corrected the mutation using PE, and generated mutation-corrected dogs by somatic cell nuclear transfer. The results indicate that PE technology can potentially be used as a platform to correct genetic defects in dogs.
犬髋关节发育不良（HD）是一种由遗传和环境因素相互作用引起的多因素疾病。HD 主要发生在中大型犬中，是一种会引起严重疼痛并需要手术干预的疾病。然而，手术并不简单，唯一的改善方法是将患有 HD 的个体犬排除在繁育计划之外。最近，基于 CRISPR-Cas9 系统的新型基因组编辑工具——Prime Editing（PE）已在植物和小鼠中得到开发和验证。在这项研究中，我们首次成功纠正了拉布拉多猎犬犬的与 HD 相关的突变。我们从被诊断患有 HD 的犬中收集细胞，使用 PE 纠正突变，并通过体细胞核转移生成突变纠正犬。结果表明，PE 技术可能可作为一种平台，用于纠正犬类的遗传缺陷。
