Khokhlov A N, Golovina M E, Chirkova E Iu, Nadzharian T L
Tsitologiia. 1987 Mar;29(3):353-7.
With the help of a previously devised model of cultured cell growth kinetics it was shown that the "plateau" level on the growth curve of human embryo diploid fibroblasts increased, if the cells were grown with Epigid geroprotector-antioxidant (equal results with two drug concentrations: 10(-5) and 10(-7) M, resp.). It was also found that the "plateau" level on the growth curve of cultured "3 days aged" Chinese hamster cells (subcultivated to a fresh medium 3 days after a 1:6 subcultivation) lays higher than the "plateau" level of "14 days aged" cells but lower than that of "7 day aged" cells. Furthermore it was shown that an increase in inoculation density of Chinese hamster cells increased proportionately the rate of cell population growth but had a little effect on the "plateau" level of the growth curve. The data obtained are discussed in terms of some "proliferative" theories of cellular ageing.
借助先前设计的培养细胞生长动力学模型表明，如果人胚二倍体成纤维细胞与表飞鸣老年保护剂-抗氧化剂一起培养（两种药物浓度分别为10(-5)和10(-7)M时结果相同），其生长曲线的“平台期”水平会升高。还发现，培养的“3日龄”中国仓鼠细胞（在1:6传代培养3天后转接至新鲜培养基）生长曲线的“平台期”水平高于“14日龄”细胞，但低于“7日龄”细胞。此外还表明，中国仓鼠细胞接种密度的增加会使细胞群体生长速率成比例增加，但对生长曲线的“平台期”水平影响较小。根据一些细胞衰老的“增殖”理论对所获得的数据进行了讨论。
