Ali Amena, Ali Abuzer, Ahmad Wasim, Amir Mohd, Ashraf Kamran, Wahab Shadma, Alam Prawez, Ahamad Adil
Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, P.O. Box 11099, Taif, 21944 Saudi Arabia.
Department of Pharmacognosy, College of Pharmacy, Taif University, P.O. Box 11099, Taif, 21944 Saudi Arabia.
3 Biotech. 2022 Sep;12(9):189. doi: 10.1007/s13205-022-03233-z. Epub 2022 Jul 25.
(spiked spiralflag ginger) is traditionally utilised for its advantages such as antidiabetic, antihyperlipidemic, diuretic, antimicrobial, and anticancer properties. However, there is no scientific evidence on the nephroprotective potential of this plant. Thus, this study tested the nephroprotective effect of the polyphenol-rich extract of leaves (PCSL) using preclinical models, including the HeK cell line and Wistar albino rats against cisplatin-induced toxicity. It also determined the polyphenolic compounds using high-performance thin-layer chromatography (HPTLC). PCSL showed significant ( < 0.05) nephroprotective potential against cisplatin-induced nephrotoxicity in HeK cells. Moreover, in vivo studies revealed significant ( < 0.05) amelioration in serum biochemical markers and antioxidant enzymes against cisplatin-induced nephrotoxicity. PCSL significantly inhibited the level of inflammatory cytokines such as TNF-α, IL-6, and IL-1β. Moreover, PCSL restored the damage of the kidney tissues and ameliorated interstitial haemorrhage, congestion in capillaries, inflammatory cell infiltration, vacuolated cytoplasm, and tubular epithelial injury with widened Bowman's space. In addition, HPTLC analysis revealed that PCSL comprised polyphenolic compounds such as caffeic acid, quercetin, and ferulic acid. In conclusion, PCSL exerted nephroprotective potential by modulating the expression of inflammation, oxidative stress, and histological architecture of kidney tissues.
The online version contains supplementary material available at 10.1007/s13205-022-03233-z.
（刺螺旋姜）传统上因其具有抗糖尿病、抗高血脂、利尿、抗菌和抗癌等特性而被利用。然而，关于这种植物的肾保护潜力尚无科学证据。因此，本研究使用包括HeK细胞系和Wistar白化大鼠在内的临床前模型，测试了刺螺旋姜叶富含多酚的提取物（PCSL）对顺铂诱导毒性的肾保护作用。研究还使用高效薄层色谱法（HPTLC）测定了多酚类化合物。PCSL对HeK细胞中顺铂诱导的肾毒性显示出显著（P＜0.05）的肾保护潜力。此外，体内研究表明，针对顺铂诱导的肾毒性，血清生化标志物和抗氧化酶有显著（P＜0.05）改善。PCSL显著抑制了炎症细胞因子如TNF-α、IL-6和IL-1β的水平。此外，PCSL修复了肾组织损伤，改善了间质出血、毛细血管充血、炎症细胞浸润、空泡化细胞质以及肾小管上皮损伤伴鲍曼间隙增宽。另外，HPTLC分析表明PCSL含有咖啡酸、槲皮素和阿魏酸等多酚类化合物。总之，PCSL通过调节炎症、氧化应激的表达以及肾组织的组织结构发挥肾保护潜力。
在线版本包含可在10.1007/s13205-022-03233-z获取的补充材料。
