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BNT162b2 mRNA 疫苗接种后心肌炎患者的外周免疫景观。

The Peripheral Immune Landscape in a Patient with Myocarditis after the Administration of BNT162b2 mRNA Vaccine.

机构信息

Department of Biochemistry and Molecular Biology, Yonsei University College of Medicine, Seoul 03722, Korea.

Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul 03722, Korea.

出版信息

Mol Cells. 2022 Oct 31;45(10):738-748. doi: 10.14348/molcells.2022.0031. Epub 2022 Jul 28.

DOI:10.14348/molcells.2022.0031
PMID:35904026
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9589374/
Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has posed a serious threat to global public health. A novel vaccine made from messenger RNA (mRNA) has been developed and approved for use at an unprecedented pace. However, an increased risk of myocarditis has been reported after BNT162b2 mRNA vaccination due to unknown causes. In this study, we used single-cell RNA sequencing and single-cell T cell receptor sequencing analyses of peripheral blood mononuclear cells (PBMCs) to describe, for the first time, changes in the peripheral immune landscape of a patient who underwent myocarditis after BNT162b2 vaccination. The greatest changes were observed in the transcriptomic profile of monocytes in terms of the number of differentially expressed genes. When compared to the transcriptome of PBMCs from vaccinated individuals without complications, increased expression levels of IL7R were detected in multiple cell clusters. Overall, results from this study can help advance research into the pathogenesis of BNT162b2-induced myocarditis.

摘要

严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 大流行对全球公共卫生构成了严重威胁。一种新型的信使 RNA(messenger RNA, mRNA) 疫苗已经被开发出来，并以前所未有的速度获得批准使用。然而，由于未知原因，在接种 BNT162b2 mRNA 疫苗后，报告的心肌炎风险增加。在这项研究中，我们使用外周血单核细胞 (peripheral blood mononuclear cells, PBMCs) 的单细胞 RNA 测序和单细胞 T 细胞受体测序分析，首次描述了 BNT162b2 接种后发生心肌炎的患者外周免疫景观的变化。在单核细胞的转录组谱方面，差异表达基因的数量发生了最大的变化。与无并发症接种个体的 PBMC 转录组相比，多个细胞簇中检测到 IL7R 的表达水平增加。总的来说，这项研究的结果可以帮助推进对 BNT162b2 诱导心肌炎发病机制的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c21/9589374/e0132910e7b7/molce-45-10-738-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c21/9589374/bec7a5de9521/molce-45-10-738-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c21/9589374/4d980e614b21/molce-45-10-738-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c21/9589374/adbb9aa88980/molce-45-10-738-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c21/9589374/c1139cdd261b/molce-45-10-738-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c21/9589374/e0132910e7b7/molce-45-10-738-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c21/9589374/bec7a5de9521/molce-45-10-738-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c21/9589374/accdc48ce82c/molce-45-10-738-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c21/9589374/4d980e614b21/molce-45-10-738-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c21/9589374/adbb9aa88980/molce-45-10-738-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c21/9589374/c1139cdd261b/molce-45-10-738-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c21/9589374/e0132910e7b7/molce-45-10-738-f6.jpg

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JCI Insight. 2021 Dec 22;6(24):e153201. doi: 10.1172/jci.insight.153201.
3
Dynamics of TCR repertoire and T cell function in COVID-19 convalescent individuals.新冠康复者TCR库及T细胞功能的动态变化
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Am J Transl Res. 2025 Mar 15;17(3):2339-2351. doi: 10.62347/CCVM6311. eCollection 2025.
4
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NPJ Vaccines. 2024 Jun 28;9(1):122. doi: 10.1038/s41541-024-00893-1.
5
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6
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7
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