promoted adipocyte differentiation by regulating gene through mA modification in Hycole rabbits.

N6-methyladenosine (mA), the most abundant internal mRNA modification in eukaryotes, plays a vital role in regulating adipogenesis. However, its underlying mechanism remains largely unknown. Our previous study found that gene has mA modification in both muscle and fat tissue. In this study, we interfered with and genes After we cultured rabbit preadipocytes respectively. Oil red O staining and triglyceride assay were used to detect adipocyte differentiation. RT-qPCR was used to detect gene expression level and MeRIP-qPCR was used to detect the mA modification level of gene. The results showed that promoted the differentiation of adipocytes. At the same time, up regulated the expression of gene and down regulated the mA modification level of gene. Finally, we found that inhibited adipocyte differentiation. Together, we showed that promoted adipocyte differentiation by regulating gene through mA modification.