Jeong Youngseok, Kang Seonmi, Ahn Junyeong, Kim Sol, Kim Hyelin, Park Jisoo, Seo Kangmoon
Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, South Korea.
Vet Ophthalmol. 2023 Apr;26 Suppl 1:89-97. doi: 10.1111/vop.13016. Epub 2022 Jul 29.
To investigate the variance in corneal epithelial thickness (CET) and limbal epithelial thickness (LET) according to the age and skull type by using spectral-domain optical coherence tomography (SD-OCT) in normal dogs.
This study used an eye each from 46 dogs (24 brachycephalic and 22 non-brachycephalic dogs) assessed to have clear corneas. Each dog was classified according to age into groups 1 (0-5 years), 2 (6-10 years), and 3 (>11 years). OCT imaging was performed on the central cornea for CET and perpendicular to the quadrant of the limbus for LET. The average of the maximum LET (maxLET) value was measured in four eye quadrants.
Corneal epithelial thickness was not significantly different according to age in brachycephalic and non-brachycephalic dogs. Visualization of the limbal invagination was better when the maxLET was thick. Nasal and temporal maxLETs were significantly thicker than superior and inferior maxLETs in non-brachycephalic dogs. In brachycephalic dogs, there was a significant decrease in nasal maxLET with age (r = -0.489, p = .015). Significant differences between brachycephalic and non-brachycephalic dogs were observed in nasal maxLET (p = .024) and temporal maxLET (p = .011).
Invagination was better visualized in the nasal and temporal limbal quadrants of non-brachycephalic dogs compared with brachycephalic dogs, and the maxLETs of the regions were thicker than those of the brachycephalic dogs. CET and LET measurements using SD-OCT can help in clinical assessment and research on ocular surface diseases in dogs.
通过使用光谱域光学相干断层扫描（SD-OCT）研究正常犬角膜上皮厚度（CET）和角膜缘上皮厚度（LET）随年龄和颅骨类型的变化。
本研究使用了46只犬（24只短头犬和22只非短头犬）的眼睛，评估角膜清晰。每只犬根据年龄分为1组（0 - 5岁）、2组（6 - 10岁）和3组（>11岁）。对中央角膜进行OCT成像测量CET，垂直于角膜缘象限测量LET。在四个眼象限测量最大LET（maxLET）值的平均值。
短头犬和非短头犬的角膜上皮厚度随年龄无显著差异。当maxLET较厚时，角膜缘内陷的可视化效果更好。在非短头犬中，鼻侧和颞侧的maxLET显著厚于上方和下方的maxLET。在短头犬中，鼻侧maxLET随年龄显著降低（r = -0.489，p = 0.015）。在鼻侧maxLET（p = 0.024）和颞侧maxLET（p = 0.011）方面，短头犬和非短头犬之间存在显著差异。
与短头犬相比，非短头犬鼻侧和颞侧角膜缘象限的内陷可视化效果更好，且这些区域的maxLET比短头犬的厚。使用SD-OCT测量CET和LET有助于犬眼表疾病的临床评估和研究。
