大肠杆菌核糖体蛋白S4的构象预测与圆二色性研究
Conformational prediction and circular dichroism studies on ribosomal protein S4 from Escherichia coli.
作者信息
Allen S H, Wong K P
出版信息
Biophys J. 1978 Sep;23(3):359-73. doi: 10.1016/S0006-3495(78)85455-1.
Abstract
The conformation of ribosomal protein S4 from Escherichia coli has been studied by circular dichroism (CD) and shown to possess unique conformation free in solution. The near ultraviolet spectrum suggests the existence of unique tertiary structural environment for the aromatic amino acid residues. The far ultraviolet spectrum gives an estimation of its secondary structure which is 32% alpha-helix and 14% beta-structure in reconstitution buffer at 25 degrees C. The conformation of S4 has been predicted from its sequence, and two models are presented here. An attempt is made to correlate these two molecular models with the available physicochemical data concerning the shape, conformation, and possible RNA binding site of protein S4.
摘要
通过圆二色性（CD）研究了来自大肠杆菌的核糖体蛋白S4的构象，结果表明其在溶液中具有独特的构象。近紫外光谱表明芳香族氨基酸残基存在独特的三级结构环境。远紫外光谱给出了其二级结构的估计值，在25℃的重构缓冲液中，其二级结构为32%的α-螺旋和14%的β-结构。已根据S4的序列预测了其构象，本文给出了两种模型。试图将这两种分子模型与有关蛋白S4的形状、构象和可能的RNA结合位点的现有物理化学数据相关联。
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Fine structure in the near-ultraviolet circular dichroism and absorption spectra of tryptophan derivatives and chymotrypsinogen A at 77 degrees K.77K下色氨酸衍生物和α-胰凝乳蛋白酶原A的近紫外圆二色性和吸收光谱中的精细结构
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Conformation of cytochromes. 3. Effect of urea, temperature, extrinsic ligands, and pH variation on the conformation of horse heart ferricytochrome c.细胞色素的构象。3. 尿素、温度、外在配体及pH变化对马心高铁细胞色素c构象的影响
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Optical rotatory dispersion and circular dichroism studies on insulin and its trypsin-modified derivatives.胰岛素及其胰蛋白酶修饰衍生物的旋光色散和圆二色性研究。
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Circular dichroism of ovine interstitial cell stimulating hormone and its subunits.绵羊促间质细胞激素及其亚基的圆二色性
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Structure and function of bacterial ribosomes. XII. Accumulation of 21 s particles by some cold-sensitive mutants of Escherichia coli.细菌核糖体的结构与功能。十二。大肠杆菌某些冷敏感突变体对21 s颗粒的积累。
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