van de Braak A E, van 't Klooster A T
Vet Res Commun. 1987;11(2):101-8. doi: 10.1007/BF00344942.
The rate of osteogenesis was studied in 8 non-pregnant, non-lactating Friesian dairy sheep, 3-6 years old, by means of a treatment with 3 different bone seeking agents. Four sheep were fed a low calcium ration (LCa:1.8 g Ca/d) and four other sheep a high calcium ration (HCa:12.7 g Ca/d). The bone markers, oxytetracycline-HCl, alizarine-complexion and demeclocycline-HCl, were administered at intervals of 6 weeks, and the sheep were killed 1 week after administration of the last marker. In undecalcified cross sections from the middle of ribs 2, 10 and 12, and from the proximal and distal parts of rib 10, the numbers of labelled osteons and the number of osteons with 1, 2 or 3 markers were counted under fluorescent microscopy. In the ribs of sheep from the LCa group, the number of labelled osteons and the quantity of labels per osteon tended to be higher than those of sheep from the HCa group. When osteogenic activity was compared in the different sites of ribs analysed, lowest osteogenic activity was observed in the proximal part of the 10th rib. The use of fluorescing markers offers the possibility of studying osteogenic activity over a certain period of time in adult sheep.
通过用3种不同的亲骨性药物进行治疗，对8只3至6岁的未怀孕、未哺乳的弗里斯兰奶羊的成骨速率进行了研究。4只羊饲喂低钙日粮（LCa：1.8克钙/天），另外4只羊饲喂高钙日粮（HCa：12.7克钙/天）。每隔6周给予骨标记物盐酸土霉素、茜素络合物和盐酸地美环素，在给予最后一种标记物1周后将羊处死。在第2、10和12肋中部以及第10肋近端和远端的未脱钙横切面上，在荧光显微镜下计数标记骨单位的数量以及带有1、2或3个标记物的骨单位数量。在LCa组羊的肋骨中，标记骨单位的数量和每个骨单位的标记物数量往往高于HCa组羊。当对所分析肋骨的不同部位的成骨活性进行比较时，在第10肋近端观察到最低的成骨活性。使用荧光标记物为研究成年羊在一段时间内的成骨活性提供了可能性。
