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通过一步绿色基于膜的纯化方法来解锁氧化辅酶 A 的获取途径。

Unlocking the access to oxidized coenzyme A via a single-step green membrane-based purification.

机构信息

URD Agro-Biotechnologies Industrielles (ABI), CEBB, AgroParisTech, 51110, Pomacle, France.

出版信息

Sci Rep. 2022 Jul 29;12(1):12991. doi: 10.1038/s41598-022-17250-8.

DOI:10.1038/s41598-022-17250-8
PMID:35906370
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9338019/
Abstract

A new membrane-based strategy to purify oxidized coenzyme A ((CoAS)) from adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) has been developed. Commercially available membranes were screened and studied (permeate flux and overall compounds retention) which allowed the identification of one efficient membrane (GK from Suez Water Technologies & Solutions). Different total compounds concentrations solutions were used in the system in order to find the following working conditions: 4 bars with a total compounds solution of 5.19 g L. Applying these conditions to a dia-filtration set-up allowed us to reach 68% pure (CoAS) in 4.8 diafiltration volumes (DV) and a 95% (CoAS) purity can be predicted in 8.5 DV. A comparative study of green metrics-i.e. process mass index (PMI)-of the classic chromatography vs the membrane-based one demonstrated the great advantages of the latter in terms of sustainability. This strategy unlocks the access to the essential and central cofactor that is coenzyme A.

摘要

已经开发出一种从三磷酸腺苷 (ATP)、二磷酸腺苷 (ADP) 和一磷酸腺苷 (AMP) 中纯化氧化辅酶 A ((CoAS)) 的新型基于膜的策略。对市售膜进行了筛选和研究（渗透通量和总体化合物保留率），从而确定了一种有效的膜（Suez Water Technologies & Solutions 的 GK）。在系统中使用不同的总化合物浓度溶液，以找到以下工作条件：4 巴，总化合物溶液为 5.19 g/L。将这些条件应用于 Dia 过滤装置，我们可以在 4.8 个 Dia 过滤体积 (DV) 中达到 68%纯度的 (CoAS)，并且可以预测在 8.5 DV 中达到 95%的 (CoAS) 纯度。经典色谱法与基于膜的方法的绿色指标——即过程质量指数 (PMI)——的比较研究表明，后者在可持续性方面具有巨大优势。该策略解锁了对必需和中心辅因子辅酶 A 的访问。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/69db5b586ae6/41598_2022_17250_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/9f0cc9531c98/41598_2022_17250_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/a3ce44ae6a34/41598_2022_17250_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/58d1c3c14e33/41598_2022_17250_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/92029aa15d22/41598_2022_17250_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/8e6e814cc201/41598_2022_17250_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/0c750b480619/41598_2022_17250_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/69db5b586ae6/41598_2022_17250_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/9f0cc9531c98/41598_2022_17250_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/a3ce44ae6a34/41598_2022_17250_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/58d1c3c14e33/41598_2022_17250_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/92029aa15d22/41598_2022_17250_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/8e6e814cc201/41598_2022_17250_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/0c750b480619/41598_2022_17250_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481b/9338019/69db5b586ae6/41598_2022_17250_Fig7_HTML.jpg

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