Unlocking the access to oxidized coenzyme A via a single-step green membrane-based purification.

A new membrane-based strategy to purify oxidized coenzyme A ((CoAS)) from adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) has been developed. Commercially available membranes were screened and studied (permeate flux and overall compounds retention) which allowed the identification of one efficient membrane (GK from Suez Water Technologies & Solutions). Different total compounds concentrations solutions were used in the system in order to find the following working conditions: 4 bars with a total compounds solution of 5.19 g L. Applying these conditions to a dia-filtration set-up allowed us to reach 68% pure (CoAS) in 4.8 diafiltration volumes (DV) and a 95% (CoAS) purity can be predicted in 8.5 DV. A comparative study of green metrics-i.e. process mass index (PMI)-of the classic chromatography vs the membrane-based one demonstrated the great advantages of the latter in terms of sustainability. This strategy unlocks the access to the essential and central cofactor that is coenzyme A.